Aptamers with Tunable Affinity Enable Single‐Molecule Tracking and Localization of Membrane Receptors on Living Cancer Cells
Abstract: Tumor cell‐surface markers are usually overexpressed or mutated protein receptors for which spatiotemporal regulation differs between and within cancers. Single‐molecule fluorescence imaging can profile individual markers in different cellular contexts with molecular precision. However, standard single‐molecule imaging methods based on overexpressed genetically encoded tags or cumbersome probes can significantly alter the native state of receptors. We introduce a live‐cell points accumulation for imaging in nanoscale topography (PAINT) method that exploits aptamers as minimally invasive affinity probes. Localization and tracking of individual receptors are based on stochastic and transient binding between aptamers and their targets. We demonstrated single‐molecule imaging of a model tumor marker (EGFR) on a panel of living cancer cells. Affinity to EGFR was finely tuned by rational engineering of aptamer sequences to define receptor motion and/or native receptor density.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Aptamers with Tunable Affinity Enable Single‐Molecule Tracking and Localization of Membrane Receptors on Living Cancer Cells ; volume:59 ; number:42 ; year:2020 ; pages:18546-18555 ; extent:10
Angewandte Chemie / International edition. International edition ; 59, Heft 42 (2020), 18546-18555 (gesamt 10)
- Creator
-
Delcanale, Pietro
Porciani, David
Pujals, Silvia
Jurkevich, Alexander
Chetrusca, Andrian
Tawiah, Kwaku D.
Burke, Donald H.
Albertazzi, Lorenzo
- DOI
-
10.1002/anie.202004764
- URN
-
urn:nbn:de:101:1-2022062213032796382569
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:21 AM CEST
Data provider
This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Delcanale, Pietro
- Porciani, David
- Pujals, Silvia
- Jurkevich, Alexander
- Chetrusca, Andrian
- Tawiah, Kwaku D.
- Burke, Donald H.
- Albertazzi, Lorenzo
Other Objects (12)
Aptamers for analytical applications : affinity acquisition and method design
High‐Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild‐Type and B.1.1.7 SARS‐CoV‐2 in Unprocessed Saliva
High‐Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild‐Type and B.1.1.7 SARS‐CoV‐2 in Unprocessed Saliva
pH‐Directed Capture‐SELEX for Nanomolar Affinity Aptamers for Kanamycin Detection
Light-Induced Switching of Tunable Single-Molecule Junctions
Light‐Induced Switching of Tunable Single‐Molecule Junctions
Photochemically responsive polymer films enable tunable gliding flights
Colloidal quantum dots enable tunable liquid-state lasers
Antibubbles Enable Tunable Payload Release with Low‐Intensity Ultrasound
CAR‐Aptamers Enable Traceless Enrichment and Monitoring of CAR‐Positive Cells and Overcome Tumor Immune Escape
Camelid V H H affinity ligands enable separation of closely related biopharmaceuticals
The α-Hemolysin nanopore transduction detector – single-molecule binding studies and immunological screening of antibodies and aptamers
Aptamers for analytical applications : affinity acquisition and method design
High‐Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild‐Type and B.1.1.7 SARS‐CoV‐2 in Unprocessed Saliva
High‐Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild‐Type and B.1.1.7 SARS‐CoV‐2 in Unprocessed Saliva
pH‐Directed Capture‐SELEX for Nanomolar Affinity Aptamers for Kanamycin Detection
Light-Induced Switching of Tunable Single-Molecule Junctions
Light‐Induced Switching of Tunable Single‐Molecule Junctions
Photochemically responsive polymer films enable tunable gliding flights
Colloidal quantum dots enable tunable liquid-state lasers
Antibubbles Enable Tunable Payload Release with Low‐Intensity Ultrasound
CAR‐Aptamers Enable Traceless Enrichment and Monitoring of CAR‐Positive Cells and Overcome Tumor Immune Escape
Camelid V H H affinity ligands enable separation of closely related biopharmaceuticals
The α-Hemolysin nanopore transduction detector – single-molecule binding studies and immunological screening of antibodies and aptamers
Aptamers for analytical applications : affinity acquisition and method design
High‐Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild‐Type and B.1.1.7 SARS‐CoV‐2 in Unprocessed Saliva
High‐Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild‐Type and B.1.1.7 SARS‐CoV‐2 in Unprocessed Saliva
pH‐Directed Capture‐SELEX for Nanomolar Affinity Aptamers for Kanamycin Detection
Light-Induced Switching of Tunable Single-Molecule Junctions
Light‐Induced Switching of Tunable Single‐Molecule Junctions
Photochemically responsive polymer films enable tunable gliding flights
Colloidal quantum dots enable tunable liquid-state lasers
Antibubbles Enable Tunable Payload Release with Low‐Intensity Ultrasound
CAR‐Aptamers Enable Traceless Enrichment and Monitoring of CAR‐Positive Cells and Overcome Tumor Immune Escape
Camelid V H H affinity ligands enable separation of closely related biopharmaceuticals