Activity Levels of Amine Transaminases Correlate with Active Site Hydrophobicity
Abstract: Amine transaminases (ATAs) are biocatalysts for the synthesis of chiral amines and can be identified in sequence databases by specific sequence motifs. This study shows that the activity level towards the model substrate 1‐phenylethylamine can be predicted solely from the sequence. To demonstrate this, 15 putative ATAs with a different distribution of hydrophobic or hydrophilic amino acid side chains near the active site were characterized. Hydrophobic side chains were associated with a high activity level and were a better predictor of activity than global sequence identity to known ATAs with high or low activities. Enzyme stability investigations revealed that four out of the 15 ATAs showed a good operational stability.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Activity Levels of Amine Transaminases Correlate with Active Site Hydrophobicity ; day:31 ; month:08 ; year:2022 ; extent:10
Chemie - Ingenieur - Technik ; (31.08.2022) (gesamt 10)
- Urheber
- DOI
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10.1002/cite.202200062
- URN
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urn:nbn:de:101:1-2022090115183337029032
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:31 MESZ
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Beteiligte
- Kollipara, Manideep
- Matzel, Philipp
- Bornscheuer, Uwe Theo
- Höhne, Matthias
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