Multiplex PCR for detection of Escherichia coli O157:H7 in foods

Abstract: Escherichia coli O157:H7 is well known enterohemorrhagic pathogen responsible for infections among animals including a man. The main source of this bacterium is cattle, that is mostly asymptomatic and through that E. coli O157:H7 can simple transfer to food products. Therefore, there is a need for rapid, sensitive and specific detection method. The present work is focused on its detection by a heptaplex polymerase chain reaction, which targets genes from known virulent regions of E. coli O157:H7. According to obtained results this approach is able to reach the detection sensitivity of 4 colony-forming units (CFU) from a culture and 6 and 8 CFU from milk and meat samples, respectively, independently of tested sample volume.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Multiplex PCR for detection of Escherichia coli O157:H7 in foods ; volume:66 ; number:3 ; year:2011 ; pages:401-405 ; extent:5 ; Biologia ; 66, Heft 3 (2011), 401-405 (gesamt 5)

Contributors, Places and Time

• Creator: Vidová, Barbora ; Tóthová, Eva ; Blahut, Ľuboslav ; Horváthová, Viera ; Godány, Andrej

Further information

• DOI: 10.2478/s11756-011-0052-z
• URN: urn:nbn:de:101:1-2409221718493.934682610700
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:25 AM CEST