Streptomyces venezuelae TX‐TL – a next generation cell‐free synthetic biology tool

Abstract: Streptomyces venezuelae is a promising chassis in synthetic biology for fine chemical and secondary metabolite pathway engineering. The potential of S. venezuelae could be further realized by expanding its capability with the introduction of its own in vitro transcription‐translation (TX‐TL) system. TX‐TL is a fast and expanding technology for bottom‐up design of complex gene expression tools, biosensors and protein manufacturing. Herein, we introduce a S. venezuelae TX‐TL platform by reporting a streamlined protocol for cell‐extract preparation, demonstrating high‐yield synthesis of a codon‐optimized sfGFP reporter and the prototyping of a synthetic tetracycline‐inducible promoter in S. venezuelae TX‐TL based on the tetO‐TetR repressor system. The aim of this system is to provide a host for the homologous production of exotic enzymes from Actinobacteria secondary metabolism in vitro. As an example, the authors demonstrate the soluble synthesis of a selection of enzymes (12–70 kDa) from the Streptomyces rimosus oxytetracycline pathway.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Streptomyces venezuelae TX‐TL – a next generation cell‐free synthetic biology tool ; volume:12 ; number:4 ; year:2017 ; extent:6 ; Biotechnology journal ; 12, Heft 4 (2017) (gesamt 6)

Contributors, Places and Time

• Creator: Moore, Simon J. ; Lai, Hung‐En ; Needham, Hannah ; Polizzi, Karen M. ; Freemont, Paul S.

Further information

• DOI: 10.1002/biot.201600678
• URN: urn:nbn:de:101:1-2022100807565234960478
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:26 AM CEST