Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes
Abstract: Circadian rhythms are cell‐autonomous biological oscillations with a period of about 24 h. Current models propose that transcriptional feedback loops are the primary mechanism for the generation of circadian oscillations. Within this framework, Drosophila S2 cells are regarded as “non‐rhythmic” cells, as they do not express several canonical circadian components. Using an unbiased multi‐omics approach, we made the surprising discovery that Drosophila S2 cells do in fact display widespread daily rhythms. Transcriptomics and proteomics analyses revealed that hundreds of genes and their products, and in particular metabolic enzymes, are rhythmically expressed in a 24‐h cycle. Metabolomics analyses extended these findings and demonstrate that central carbon metabolism and amino acid metabolism are core metabolic pathways driven by protein rhythms. We thus demonstrate that 24‐h metabolic oscillations, coupled to gene and protein cycles, take place in nucleated cells without the contribution of any known circadian regulators. These results therefore suggest a reconsideration of existing models of the clockwork in Drosophila and other eukaryotic systems.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes ; volume:14 ; number:8 ; year:2018 ; extent:11
Molecular systems biology ; 14, Heft 8 (2018) (gesamt 11)
- Creator
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Rey, Guillaume
Milev, Nikolay B.
Valekunja, Utham K.
Ch, Ratnasekhar
Ray, Sandipan
Silva Dos Santos, Mariana
Nagy, Andras D.
Antrobus, Robin
MacRae, James I.
Reddy, Akhilesh B.
- DOI
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10.15252/msb.20188376
- URN
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urn:nbn:de:101:1-2022091007435370795772
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:24 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Rey, Guillaume
- Milev, Nikolay B.
- Valekunja, Utham K.
- Ch, Ratnasekhar
- Ray, Sandipan
- Silva Dos Santos, Mariana
- Nagy, Andras D.
- Antrobus, Robin
- MacRae, James I.
- Reddy, Akhilesh B.
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