Nano-characterization of Jagged-1-educated dendritic cells

Abstract: Jagged-1-educated dendritic cells (DCs) are of distinct phenotypes different from Lipopolysaccharide (LPS)-educated DCs. However, to date, little is known as to nano-features of Jagged-1-educated DCs. In this study, nanostructure of Jagged-1-educated DCs was observed through atomic force microscopy. Our results showed that the volume, surface area and width of Jagged-1-educated DCs, and the number of protrusion, pseudopodia and lamellapodia on the surface of Jagged-1-educated DCs were significantly more than those of GM-CSF-induced DCs, but less than those of LPS-educated DCs. Compared with GM-CSF-induced DCs, the roughness on the surface of Jagged-1-educated DCs was greatly increased, similar to LPS-educated DCs, but the particle size and number of them on the membrane were markedly less than the latter. The change of DC nanostructure caused by Jagged-1 was abrogated with N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycinet-butyl ester, a γ-secretase inhibitor of Notch signaling pathway. Compared to LPS-educated DCs, Jagged-1-educated DCs highly expressed NICD, Deltex-1 and Hes-1 proteins, moderately produced IL-12, but not IFN-γ, and the level of CD40 molecules on the surface of them was much lower, suggesting that Jagged-1-educated DCs are in a semi-mature status and may have unique functions distinguished from LPS-educated DCs.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Nano-characterization of Jagged-1-educated dendritic cells ; volume:6 ; number:6 ; year:2011 ; pages:981-989 ; extent:9 ; Open life sciences ; 6, Heft 6 (2011), 981-989 (gesamt 9)

Beteiligte, Orts- und Zeitangaben

• Urheber: Xing, Feiyue ; Wang, Jiongkun ; Fang, Zhiyuan ; Hu, Mingqian ; Yu, Yu ; Chen, Guoliang ; Liu, Jing

Weitere Informationen

• DOI: 10.2478/s11535-011-0063-3
• URN: urn:nbn:de:101:1-2409201856224.214163610367
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:27 MESZ