Semi-automated detection of fractional shortening in zebrafish embryo heart videos

Abstract: Quantifying cardiac functions in model organisms like embryonic zebrafish is of high importance in small molecule screens for new therapeutic compounds. One relevant cardiac parameter is the fractional shortening (FS). A method for semi-automatic quantification of FS in video recordings of zebrafish embryo hearts is presented. The software provides automated visual information about the end-systolic and end-diastolic stages of the heart by displaying corresponding colored lines into a Motion-mode display. After manually marking the ventricle diameters in frames of end-systolic and end-diastolic stages, the FS is calculated. The software was evaluated by comparing the results of the determination of FS with results obtained from another established method. Correlations of 0.96 < r < 0.99 between the two methods were found indicating that the new software provides comparable results for the determination of the FS.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Semi-automated detection of fractional shortening in zebrafish embryo heart videos ; volume:2 ; number:1 ; year:2016 ; pages:233-236 ; extent:4 ; Current directions in biomedical engineering ; 2, Heft 1 (2016), 233-236 (gesamt 4)

Contributors, Places and Time

• Creator: Nasrat, Sara ; Marcato, Daniel ; Hirth, Sofia ; Reischl, Markus ; Pylatiuk, Christian

Further information

• DOI: 10.1515/cdbme-2016-0052
• URN: urn:nbn:de:101:1-2410141643304.737828050877
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:26 AM CEST