Nanoplastics: Immune Impact, Detection, and Internalization after Human Blood Exposure by Single‐Cell Mass Cytometry

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Abstract: The increasing exposure to nanoplastics (NPs) raises significant concerns for human health, primarily due to their potential bioaccumulative properties. While NPs have recently been detected in human blood, their interactions with specific immune cell subtypes and their impact on immune regulation remain unclear. In this proof‐of‐concept study, model palladium‐doped polystyrene NPs (PS‐Pd NPs) are utilized to enable single‐cell mass cytometry (CyTOF) detection. The size‐dependent impact of carboxylate polystyrene NPs (50–200 nm) is investigated across 15 primary immune cell subpopulations using CyTOF. By taking advantage of Pd‐doping for detecting PS‐Pd NPs, this work evaluates their impact on human immune‐cells at the single‐cell level following blood exposure. This work traces PS‐Pd NPs in 37 primary immune‐cell subpopulations from human blood, quantifying the palladium atom count per cell by CyTOF while simultaneously assessing the impact of PS‐Pd NPs on cell viability, functionality, and uptake. These results demonstrate that NPs can interact with, interfere with, and translocate into several immune cell subpopulations after exposure. In vivo distribution experiments in mice further confirmed their accumulation in immune cells within the liver, blood, and spleen, particularly in monocytes, macrophages, and dendritic cells. These findings provide valuable insights into the impact of NPs on human health.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Nanoplastics: Immune Impact, Detection, and Internalization after Human Blood Exposure by Single‐Cell Mass Cytometry ; day:24 ; month:10 ; year:2024 ; extent:18
Advanced materials ; (24.10.2024) (gesamt 18)

Creator: Fusco, Laura
Gazzi, Arianna
Giro, Linda
Schefer, Roman B.
D'Almeida, Sènan Mickael
Cagliani, Roberta
Zoccheddu, Martina
Uyar, Recep
Besbinar, Ömur
Çelik, Doğantan
Yilmazer, Acelya
Mitrano, Denise M.
Orecchioni, Marco
Delogu, Lucia Gemma

DOI: 10.1002/adma.202413413

URN: urn:nbn:de:101:1-2410251418211.705225286702

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 14.02.2025, 12:39 AM CET

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Fusco, Laura
Gazzi, Arianna
Giro, Linda
Schefer, Roman B.
D'Almeida, Sènan Mickael
Cagliani, Roberta
Zoccheddu, Martina
Uyar, Recep
Besbinar, Ömur
Çelik, Doğantan
Yilmazer, Acelya
Mitrano, Denise M.
Orecchioni, Marco
Delogu, Lucia Gemma

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Nanoplastics: Immune Impact, Detection, and Internalization after Human Blood Exposure by Single‐Cell Mass Cytometry

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