EPR Distance Measurements on Long Non‐coding RNAs Empowered by Genetic Alphabet Expansion Transcription

Abstract: We present herein a novel nitroxide spin label‐containing RNA triphosphate TPT3NO and its application for site‐specific spin‐labeling of RNA through in vitro transcription using an expanded genetic alphabet. Our strategy allows the facile preparation of spin‐labeled RNAs with sizes ranging from short RNA oligonucleotides to large, complex RNA molecules with over 370 nucleotides by standard in vitro transcription. As a proof of concept, inter‐spin distance distributions are measured by pulsed electron paramagnetic resonance (EPR) spectroscopy in short self‐complementary RNA sequences and in a well‐studied 185 nucleotide non‐coding RNA, the B. subtilis glmS ribozyme. The approach is then applied to probe for the first time the folding of the 377 nucleotide A‐region of the long non‐coding RNA Xist, by PELDOR.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: EPR Distance Measurements on Long Non‐coding RNAs Empowered by Genetic Alphabet Expansion Transcription ; volume:59 ; number:20 ; year:2020 ; pages:7891-7896 ; extent:6 ; Angewandte Chemie / International edition. International edition ; 59, Heft 20 (2020), 7891-7896 (gesamt 6)

Beteiligte, Orts- und Zeitangaben

• Urheber: Domnick, Christof ; Eggert, Frank ; Wuebben, Christine ; Bornewasser, Lisa ; Hagelueken, Gregor ; Schiemann, Olav ; Kath‐Schorr, Stephanie

Weitere Informationen

• DOI: 10.1002/anie.201916447
• URN: urn:nbn:de:101:1-2022061212192836611266
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:27 MESZ