Application of Blood Group Genotyping by Next-Generation Sequencing in Various Immunohaematology Cases
Background: Next-generation sequencing (NGS) technology has been recently introduced into blood group genotyping; however, there are few studies using NGS-based blood group genotyping in real-world clinical settings. In this study, we applied NGS-based blood group genotyping into various immunohaematology cases encountered in routine clinical practice. Methods: This study included 4 immunohaematology cases: ABO subgroup, ABO chimerism, antibody to a high-frequency antigen (HFA), and anti-CD47 interference. We designed a hybridization capture-based NGS panel targeting 39 blood group-related genes and applied it to the 4 cases. Results: NGS analysis revealed a novel intronic variant (NM_020469.3:c.29-10T>G) in a patient with an Ael phenotype and detected a small fraction of ABO*A1.02 (approximately 3–6%) coexisting with the major genotype ABO*B.01/O.01.02 in dizygotic twins. In addition, NGS analysis found a homozygous stop-gain variant (NM_004827.3:c.376C>T, p.Gln126*; ABCG2*01N.01) in a patient with an antibody to an HFA; consequently, this patient’s phenotype was predicted as Jr (a−). Lastly, blood group phenotypes predicted by NGS were concordant with those determined by serology in 2 patients treated with anti-CD47 drugs. Conclusion: NGS-based blood group genotyping can be used for identifying ABO subgroup alleles, low levels of blood group chimerism, and antibodies to HFAs. Furthermore, it can be applied to extended blood group antigen matching for patients treated with anti-CD47 drugs.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Application of Blood Group Genotyping by Next-Generation Sequencing in Various Immunohaematology Cases ; volume:49 ; number:2 ; year:2021 ; pages:88-97 ; extent:10
Transfusion medicine and hemotherapy ; 49, Heft 2 (2021), 88-97 (gesamt 10)
- Urheber
-
Kim, Tae Yeul
Yu, HongBi
Phan, Minh-Trang Thi
Jang, Ja-Hyun
Cho, Duck
- DOI
-
10.1159/000517565
- URN
-
urn:nbn:de:101:1-2022041400195523919451
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:32 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Kim, Tae Yeul
- Yu, HongBi
- Phan, Minh-Trang Thi
- Jang, Ja-Hyun
- Cho, Duck
Ähnliche Objekte (12)
Genotyping 1000 yeast strains by next-generation sequencing
HLA genotyping by next-generation sequencing of complementary DNA
HLAscan: genotyping of the HLA region using next-generation sequencing data
Next-Generation Sequencing Data Analysis
The mapping task and its various applications in next-generation sequencing
Limitations in next-generation sequencing-based genotyping of breast cancer polygenic risk score loci
High-throughput multiplex HLA genotyping by next-generation sequencing using multi-locus individual tagging
High-throughput polymorphism detection and genotyping in Brassica napus using next-generation RAD sequencing
Next-generation sequencing in plant systematics
Next-generation sequencing at Merck-Boston
Next-generation sequencing in thyroid cancer
Special issue next generation sequencing and genotyping
Genotyping 1000 yeast strains by next-generation sequencing
HLA genotyping by next-generation sequencing of complementary DNA
HLAscan: genotyping of the HLA region using next-generation sequencing data
Next-Generation Sequencing Data Analysis
The mapping task and its various applications in next-generation sequencing
Limitations in next-generation sequencing-based genotyping of breast cancer polygenic risk score loci
High-throughput multiplex HLA genotyping by next-generation sequencing using multi-locus individual tagging
High-throughput polymorphism detection and genotyping in Brassica napus using next-generation RAD sequencing
Next-generation sequencing in plant systematics
Next-generation sequencing at Merck-Boston
Next-generation sequencing in thyroid cancer
Special issue next generation sequencing and genotyping
Genotyping 1000 yeast strains by next-generation sequencing
HLA genotyping by next-generation sequencing of complementary DNA
HLAscan: genotyping of the HLA region using next-generation sequencing data
Next-Generation Sequencing Data Analysis
The mapping task and its various applications in next-generation sequencing
Limitations in next-generation sequencing-based genotyping of breast cancer polygenic risk score loci
High-throughput multiplex HLA genotyping by next-generation sequencing using multi-locus individual tagging
High-throughput polymorphism detection and genotyping in Brassica napus using next-generation RAD sequencing
Next-generation sequencing in plant systematics
Next-generation sequencing at Merck-Boston
Next-generation sequencing in thyroid cancer