CLK2 Condensates Reorganize Nuclear Speckles and Induce Intron Retention
Abstract: Intron retention (IR) constitutes a less explored form of alternative splicing, wherein introns are retained within mature mRNA transcripts. This investigation demonstrates that the cell division cycle (CDC)‐like kinase 2 (CLK2) undergoes liquid–liquid phase separation (LLPS) within nuclear speckles in response to heat shock (HS). The formation of CLK2 condensates depends on the intrinsically disordered region (IDR) located within the N‐terminal amino acids 1‐148. Phosphorylation at residue T343 sustains CLK2 kinase activity and promotes overall autophosphorylation, which inhibits the LLPS activity of the IDR. These CLK2 condensates initiate the reorganization of nuclear speckles, transforming them into larger, rounded structures. Moreover, these condensates facilitate the recruitment of splicing factors into these compartments, restricting their access to mRNA for intron splicing and promoting the IR. The retained introns lead to the sequestration of transcripts within the nucleus. These findings extend to the realm of glioma stem cells (GSCs), where a physiological state mirroring HS stress inhibits T343 autophosphorylation, thereby inducing the formation of CLK2 condensates and subsequent IR. Notably, expressing the CLK2 condensates hampers the maintenance of GSCs. In conclusion, this research unveils a mechanism by which IR is propelled by CLK2 condensates, shedding light on its role in coping with cellular stress.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
CLK2 Condensates Reorganize Nuclear Speckles and Induce Intron Retention ; day:09 ; month:08 ; year:2024 ; extent:17
Advanced science ; (09.08.2024) (gesamt 17)
- Creator
-
Wang, Bing
Li, Jing
Song, Yanyang
Qin, Xuhui
Lu, Xia
Huang, Wei
Peng, Chentai
Wei, Jinxia
Huang, Donghui
Wang, Wei
- DOI
-
10.1002/advs.202309588
- URN
-
urn:nbn:de:101:1-2408091437230.988204148052
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
14.08.2025, 10:48 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Wang, Bing
- Li, Jing
- Song, Yanyang
- Qin, Xuhui
- Lu, Xia
- Huang, Wei
- Peng, Chentai
- Wei, Jinxia
- Huang, Donghui
- Wang, Wei
Other Objects (12)
Speech recognition using an english multimodal corpus with integrated image and depth information
How does the one child policy impact social and economic outcomes?
Understanding the Effects of Education on Health: Evidence from China
Auditor choice and accruals patterns of cross-listed firms
Crystal structure of 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene- N, N ′-bis (1,3-bis (2,6-diisopropylphenyl)-1,3-dihydro-2 H -1,3,2-diazaborol-2-yl)- l 2 -germenediamine, C 63 H 94 B 2 GeN 8
RETRACTED ARTICLE: Investigation of the hub genes and related mechanism in ovarian cancer via bioinformatics analysis
Retraction Note: Investigation of the hub genes and related mechanism in ovarian cancer via bioinformatics analysis
Effects of Fuel Concentration Gradient on Stabilization of Oblique Detonation Waves in Kerosene–Air Mixtures
Effects of mulberry leaf silage on antioxidant and immunomodulatory activity and rumen bacterial community of lambs
Long non-coding RNA TUG1 aggravates cerebral ischemia and reperfusion injury by sponging miR-493-3p/miR-410-3p
The First Total Synthesis of Sphingofungin E and the Determination of its Stereochemistry
The crystal structure of 1,4-bis (1,2,3,4,5-pentamethylcyclopenta-2,4-dien-1-yl)-3,6-bis ((4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl) methylene)-1,4-dialuminacyclohexane – benzene (1/2), C 50 H 72 Al 2 B 2 O 4
Speech recognition using an english multimodal corpus with integrated image and depth information
How does the one child policy impact social and economic outcomes?
Understanding the Effects of Education on Health: Evidence from China
Auditor choice and accruals patterns of cross-listed firms
Crystal structure of 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene- N, N ′-bis (1,3-bis (2,6-diisopropylphenyl)-1,3-dihydro-2 H -1,3,2-diazaborol-2-yl)- l 2 -germenediamine, C 63 H 94 B 2 GeN 8
RETRACTED ARTICLE: Investigation of the hub genes and related mechanism in ovarian cancer via bioinformatics analysis
Retraction Note: Investigation of the hub genes and related mechanism in ovarian cancer via bioinformatics analysis
Effects of Fuel Concentration Gradient on Stabilization of Oblique Detonation Waves in Kerosene–Air Mixtures
Effects of mulberry leaf silage on antioxidant and immunomodulatory activity and rumen bacterial community of lambs
Long non-coding RNA TUG1 aggravates cerebral ischemia and reperfusion injury by sponging miR-493-3p/miR-410-3p
The First Total Synthesis of Sphingofungin E and the Determination of its Stereochemistry
The crystal structure of 1,4-bis (1,2,3,4,5-pentamethylcyclopenta-2,4-dien-1-yl)-3,6-bis ((4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl) methylene)-1,4-dialuminacyclohexane – benzene (1/2), C 50 H 72 Al 2 B 2 O 4
Speech recognition using an english multimodal corpus with integrated image and depth information
How does the one child policy impact social and economic outcomes?
Understanding the Effects of Education on Health: Evidence from China
Auditor choice and accruals patterns of cross-listed firms
Crystal structure of 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene- N, N ′-bis (1,3-bis (2,6-diisopropylphenyl)-1,3-dihydro-2 H -1,3,2-diazaborol-2-yl)- l 2 -germenediamine, C 63 H 94 B 2 GeN 8
RETRACTED ARTICLE: Investigation of the hub genes and related mechanism in ovarian cancer via bioinformatics analysis
Retraction Note: Investigation of the hub genes and related mechanism in ovarian cancer via bioinformatics analysis
Effects of Fuel Concentration Gradient on Stabilization of Oblique Detonation Waves in Kerosene–Air Mixtures
Effects of mulberry leaf silage on antioxidant and immunomodulatory activity and rumen bacterial community of lambs
Long non-coding RNA TUG1 aggravates cerebral ischemia and reperfusion injury by sponging miR-493-3p/miR-410-3p
The First Total Synthesis of Sphingofungin E and the Determination of its Stereochemistry