The disulfide catalyst QSOX1 maintains the colon mucosal barrier by regulating Golgi glycosyltransferases

Abstract: Mucus is made of enormous mucin glycoproteins that polymerize by disulfide crosslinking in the Golgi apparatus. QSOX1 is a catalyst of disulfide bond formation localized to the Golgi. Both QSOX1 and mucins are highly expressed in goblet cells of mucosal tissues, leading to the hypothesis that QSOX1 catalyzes disulfide‐mediated mucin polymerization. We found that knockout mice lacking QSOX1 had impaired mucus barrier function due to production of defective mucus. However, an investigation on the molecular level revealed normal disulfide‐mediated polymerization of mucins and related glycoproteins. Instead, we detected a drastic decrease in sialic acid in the gut mucus glycome of the QSOX1 knockout mice, leading to the discovery that QSOX1 forms regulatory disulfides in Golgi glycosyltransferases. Sialylation defects in the colon are known to cause colitis in humans. Here we show that QSOX1 redox control of sialylation is essential for maintaining mucosal function.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: The disulfide catalyst QSOX1 maintains the colon mucosal barrier by regulating Golgi glycosyltransferases ; day:17 ; month:10 ; year:2022 ; extent:15 ; The EMBO journal / European Molecular Biology Organization ; (17.10.2022) (gesamt 15)

Beteiligte, Orts- und Zeitangaben

• Urheber: Ilani, Tal ; Reznik, Nava ; Yeshaya, Noa ; Feldman, Tal ; Vilela, Patrick ; Lansky, Zipora ; Javitt, Gabriel ; Shemesh, Michal ; Brenner, Ori ; Elkis, Yoav ; Varsano, Neta ; Jaramillo, Ana M. ; Evans, Christopher M. ; Fass, Deborah

Weitere Informationen

• DOI: 10.15252/embj.2022111869
• URN: urn:nbn:de:101:1-2022101715011593835674
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:23 MESZ