Cellular Architects at Work: Cells Building their Own Microgel Houses
Abstract: Microporous annealed particle (MAP) scaffolds are investigated for their application as injectable 3D constructs in the field of regenerative medicine and tissue repair. While available MAP scaffolds provide a stable interlinked matrix of microgels for cell culture, the infiltration depth and space for cells to grow inside the scaffolds is pre‐determined by the void fraction during the assembly. In the case of MAP scaffolds fabricated from interlinked spherical microgels, a cellularity gradient can be observed with the highest cell density on the scaffold surface. Additionally, the interlinked microgel network limits the ability of cells to remodel their environment, which contradicts native tissue dynamics. In this work, a cell‐induced interlinking method for MAP scaffold formation is established, which avoids the necessity of chemical crosslinkers and pre‐engineered pores to achieve micro‐ or macropores in these 3D frameworks. This method enables cells to self‐organize with microgels into dynamic tissue constructs, which can be further controlled by altering the microgel properties, the cell/microgel ratio, and well shape. To form a cell‐induced interlinked scaffold, the cells are mixed with dextran‐based microgels and function as a glue between the microgels, resulting in a more homogenous cell distribution throughout the scaffold with efficient cell–cell interactions.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Cellular Architects at Work: Cells Building their Own Microgel Houses ; day:28 ; month:11 ; year:2023 ; extent:12
Advanced healthcare materials ; (28.11.2023) (gesamt 12)
- Creator
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Bulut, Selin
Günther, Daniel
Bund, Michelle
Haats, Christina
Bissing, Thomas
Bastard, Céline
Wessling, Matthias
De Laporte, Laura
Pich, Andrij
- DOI
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10.1002/adhm.202302957
- URN
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urn:nbn:de:101:1-2023112914250602659991
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:35 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Bulut, Selin
- Günther, Daniel
- Bund, Michelle
- Haats, Christina
- Bissing, Thomas
- Bastard, Céline
- Wessling, Matthias
- De Laporte, Laura
- Pich, Andrij
Other Objects (12)
Cellular Architects at Work : Cells Building their Own Microgel Houses
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High-Throughput Production of Micrometer Sized Double Emulsions and Microgel Capsules in Parallelized 3D Printed Microfluidic Devices
Continuous production of functional microgel building blocks for biocompatible 3D scaffolds
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Digitally Fabricated and Naturally Augmented In Vitro Tissues
Ion transport through microgel-modified membrane surfaces
Cellular Architects at Work : Cells Building their Own Microgel Houses
Fiber spinning for tissue engineering applications
Electrical impedance spectroscopy on biological barriers
Stimuli responsive porous scaffold systems for physiological cell expansion and tissue engineering applications
Wet spinning of core-shell and hollow polyelectrolyte fibers
Microgel-based Regenerative Materials and Biofunctionalization
Functional microgel coatings
High-Throughput Production of Micrometer Sized Double Emulsions and Microgel Capsules in Parallelized 3D Printed Microfluidic Devices
Continuous production of functional microgel building blocks for biocompatible 3D scaffolds
Development of in situ assembling microgel-in-hydrogel matrices for directed spinal cord regeneration
Digitally Fabricated and Naturally Augmented In Vitro Tissues
Ion transport through microgel-modified membrane surfaces
Cellular Architects at Work : Cells Building their Own Microgel Houses
Fiber spinning for tissue engineering applications
Electrical impedance spectroscopy on biological barriers
Stimuli responsive porous scaffold systems for physiological cell expansion and tissue engineering applications
Wet spinning of core-shell and hollow polyelectrolyte fibers
Microgel-based Regenerative Materials and Biofunctionalization
Functional microgel coatings
High-Throughput Production of Micrometer Sized Double Emulsions and Microgel Capsules in Parallelized 3D Printed Microfluidic Devices
Continuous production of functional microgel building blocks for biocompatible 3D scaffolds
Development of in situ assembling microgel-in-hydrogel matrices for directed spinal cord regeneration
Digitally Fabricated and Naturally Augmented In Vitro Tissues