Bioengineering scalable and drug-responsive in vitro human multicellular non-alcoholic fatty liver disease microtissues encapsulated in the liver extracellular matrix-derived hydrogel
Abstract: Non-alcoholic fatty liver disease (NAFLD) is a high-prevalence and progressive disorder. Due to lack of reliable in vitro models to recapitulate the consecutive phases, the exact pathogenesis mechanism of this disease and approved therapeutic medications have not been revealed yet. It has been proven that the interplay between multiple hepatic cell types and liver extracellular matrix (ECM) are critical in NAFLD initiation and progression. Herein, a liver microtissue (LMT) consisting of Huh-7, THP-1, and LX-2 cell lines and human umbilical vein endothelial cells (HUVEC), which could be substituted for the main hepatic cells (hepatocyte, Kupffer, stellate, and sinusoidal endothelium, respectively), encapsulated in liver derived ECM-Alginate composite, was bioengineered. When the microtissues were treated with free fatty acids (FFAs) including Oleic acid (6.6×10−4M) and Palmitic acid (3.3×10−4M), they displayed the key features of NAFLD, including similar pattern of transcripts for g.... https://www.excli.de/index.php/excli/article/view/6878
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Bioengineering scalable and drug-responsive in vitro human multicellular non-alcoholic fatty liver disease microtissues encapsulated in the liver extracellular matrix-derived hydrogel ; volume:23 ; year:2024
EXCLI journal ; 23 (2024)
- Urheber
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Asadollahi, Negar
Hajari, Mohammad Amin
Alipour Choshali, Mahmoud
Ajoudanian, Mohammad
Ziai, Seyed Ali
Vosough, Massoud
Piryaei, Abbas
- DOI
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10.17179/excli2023-6878
- URN
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urn:nbn:de:101:1-2406210313478.615470843458
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 10:50 MESZ
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Beteiligte
- Asadollahi, Negar
- Hajari, Mohammad Amin
- Alipour Choshali, Mahmoud
- Ajoudanian, Mohammad
- Ziai, Seyed Ali
- Vosough, Massoud
- Piryaei, Abbas
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