Embedded Bioprinting of Breast Tumor Cells and Organoids Using Low‐Concentration Collagen‐Based Bioinks
Abstract: Bioinks for 3D bioprinting of tumor models should not only meet printability requirements but also accurately maintain and support phenotypes of tumor surrounding cells to recapitulate key tumor hallmarks. Collagen is a major extracellular matrix protein for solid tumors, but low viscosity of collagen solution has made 3D bioprinted cancer models challenging. This work produces embedded, bioprinted breast cancer cells and tumor organoid models using low‐concentration collagen I based bioinks. The biocompatible and physically crosslinked silk fibroin hydrogel is used to generate the support bath for the embedded 3D printing. The composition of the collagen I based bioink is optimized with a thermoresponsive hyaluronic acid‐based polymer to maintain the phenotypes of both the noninvasive epithelial and invasive breast cancer cells, as well as cancer‐associated fibroblasts. Mouse breast tumor organoids are bioprinted using optimized collagen bioink to mimic in vivo tumor morphology. A vascularized tumor model is also created using a similar strategy, with significantly enhanced vasculature formation under hypoxia. This study shows the great potential of embedded bioprinted breast tumor models utilizing a low‐concentration collagen‐based bioink for advancing the understanding of tumor cell biology and facilitating drug discovery research.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Embedded Bioprinting of Breast Tumor Cells and Organoids Using Low‐Concentration Collagen‐Based Bioinks ; day:14 ; month:07 ; year:2023 ; extent:16
Advanced healthcare materials ; (14.07.2023) (gesamt 16)
- Urheber
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Shi, Wen
Mirza, Sameer
Kuss, Mitchell
Liu, Bo
Hartin, Andrew
Wan, Shibiao
Kong, Yunfan
Mohapatra, Bhopal
Krishnan, Mena
Band, Hamid
Band, Vimla
Duan, Bin
- DOI
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10.1002/adhm.202300905
- URN
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urn:nbn:de:101:1-2023071415302476928578
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 10:47 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Shi, Wen
- Mirza, Sameer
- Kuss, Mitchell
- Liu, Bo
- Hartin, Andrew
- Wan, Shibiao
- Kong, Yunfan
- Mohapatra, Bhopal
- Krishnan, Mena
- Band, Hamid
- Band, Vimla
- Duan, Bin
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