hnRNP-K Targets Open Chromatin in Mouse Embryonic Stem Cells in Concert with Multiple Regulators

Abstract: The transcription factor Oct4 plays a key regulatory role in the induction and maintenance of cellular pluripotency. In this article, we show that ubiquitous and multifunctional poly(C) DNA/RNA-binding protein hnRNP-K occupies Oct4 (Pou5f1) enhancers in embryonic stem cells (ESCs) but is dispensable for the initiation, maintenance, and downregulation of Oct4 gene expression. Nevertheless, hnRNP-K has an essential cell-autonomous function in ESCs to maintain their proliferation and viability. To better understand mechanisms of hnRNP-K action in ESCs, we have performed ChIP-seq analysis of genome-wide binding of hnRNP-K and identified several thousands of hnRNP-K target sites that are frequently co-occupied by pluripotency-related and common factors (Oct4, TATA-box binding protein, Sox2, Nanog, Otx2, etc.), as well as active histone marks. Furthermore, hnRNP-K localizes exclusively within open chromatin, implying its role in the onset and/or maintenance of this chromatin state. Stem Cells 2019;37:1018–1029

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: Stem cells. - 37, 8 (2019) , 1018-1029, ISSN: 1549-4918

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2022
• Creator: Bakhmet, Evgeny I. ; Nazarov, Igor B. ; Gazizova, Adel R. ; Vorobyeva, Nadezhda E. ; Kuzmin, Andrey A. ; Gordeev, Mikhail N. ; Sinenko, Sergey A. ; Aksenov, Nikolai D. ; Artamonova, Tatyana O. ; Khodorkovskii, Mikhail A. ; Alenina, Natalia ; Onichtchouk, Daria ; Wu, Guangming ; Schöler, Hans Robert ; Tomilin, Alexey N.
• Contributor: Albert-Ludwigs-Universität Freiburg. Entwicklungsbiologie

Further information

• DOI: 10.1002/stem.3025
• URN: urn:nbn:de:bsz:25-freidok-2298630
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:49 PM CET