Exploiting spatial dimensions to enable parallelized continuous directed evolution
Abstract: Current strategies to improve the throughput of continuous directed evolution technologies often involve complex mechanical fluid‐controlling system or robotic platforms, which limits their popularization and application in general laboratories. Inspired by our previous study on bacterial range expansion, in this study, we report a system termed SPACE for rapid and extensively parallelizable evolution of biomolecules by introducing spatial dimensions into the landmark phage‐assisted continuous evolution system. Specifically, M13 phages and chemotactic Escherichia coli cells were closely inoculated onto a semisolid agar. The phages came into contact with the expanding front of the bacterial range, and then comigrated with the bacteria. This system leverages competition over space, wherein evolutionary progress is closely associated with the production of spatial patterns, allowing the emergence of improved or new protein functions. In a prototypical problem, SPACE remarkably simplified the process and evolved the promoter recognition of T7 RNA polymerase (RNAP) to a library of 96 random sequences in parallel. These results establish SPACE as a simple, easy to implement, and massively parallelizable platform for continuous directed evolution in general laboratories.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Exploiting spatial dimensions to enable parallelized continuous directed evolution ; volume:18 ; number:9 ; year:2022 ; extent:17
Molecular systems biology ; 18, Heft 9 (2022) (gesamt 17)
- Creator
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Wei, Ting
Lai, Wangsheng
Chen, Qian
Zhang, Yi
Sun, Chenjian
He, Xionglei
Zhao, Guoping
Fu, Xiongfei
Liu, Chenli
- DOI
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10.15252/msb.202210934
- URN
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urn:nbn:de:101:1-2022092115195490480239
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:37 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Wei, Ting
- Lai, Wangsheng
- Chen, Qian
- Zhang, Yi
- Sun, Chenjian
- He, Xionglei
- Zhao, Guoping
- Fu, Xiongfei
- Liu, Chenli
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