The Impact of Zinc on Manganese Bioavailability and Cytotoxicity in HepG2 Cells
Scope: Despite their essentiality, several studies have shown that either manganese (Mn) or zinc (Zn) overexposure may lead to detrimental health effects. Although Mn is transported by some of the SLC family transporters that translocate Zn, the role of Zn in hepatocellular Mn transport and Mn‐induced toxicity have yet to be fully characterized. Methods and Results: The human hepatoma cell line, HepG2, is utilized. Total cellular Mn and Zn amounts are determined after cells are treated with Zn 2 or 24 h prior to Mn incubation for additional 24 h with inductively coupled plasma‐based spectrometry and labile Zn is assessed with the fluorescent probe FluoZin‐3. Furthermore, mRNA expression of genes involved in metal homeostasis, and mechanistic endpoints associated with Mn‐induced cytotoxicity are addressed. These results suggest that Zn protects against Mn‐induced cytotoxicity and impacts Mn bioavailability to a great extent when cells are preincubated with higher Zn concentrations for longer duration as characterized by decreased activation of caspase‐3 as well as lactate dehydrogenase (LDH) release. Conclusions: Zn protects against Mn‐induced cytotoxicity in HepG2 cells possibly due to decreased Mn bioavailability. Additionally, mRNA expression of metal homeostasis‐related genes indicates possible underlying pathways that should to be addressed in future studies.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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The Impact of Zinc on Manganese Bioavailability and Cytotoxicity in HepG2 Cells ; day:09 ; month:02 ; year:2023 ; extent:11
Molecular nutrition & food research ; (09.02.2023) (gesamt 11)
- Creator
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Michaelis, Vivien
Kasper, Silja
Naperkowski, Lisa
Pusse, Jan
Thiel, Alicia
Ebert, Franziska
Aschner, Michael
Schwerdtle, Tanja
Haase, Hajo
Bornhorst, Julia
- DOI
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10.1002/mnfr.202200283
- URN
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urn:nbn:de:101:1-2023021014202888466507
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:58 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Michaelis, Vivien
- Kasper, Silja
- Naperkowski, Lisa
- Pusse, Jan
- Thiel, Alicia
- Ebert, Franziska
- Aschner, Michael
- Schwerdtle, Tanja
- Haase, Hajo
- Bornhorst, Julia
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The Impact of Zinc on Manganese Bioavailability and Cytotoxicity in HepG2 Cells
Involvement of mitochondrial pathway in NCTD-induced cytotoxicity in human hepG2 cells
Artemyrianins A–G from Artemisia myriantha and Their Cytotoxicity Against HepG2 Cells
Comparison of different polar compounds-induced cytotoxicity in human hepatocellular carcinoma HepG2 cells
An in vitro cytotoxicity of glufosfamide in HepG2 cells relative to its nonconjugated counterpart
Synthesis and in vitro cytotoxicity evaluation of isatin-pyrrole derivatives against HepG2 cell line
Pharmakologisch purinerge Modulation des Sauerstoffverbrauchs von HEP-G2 Zellen
Characterization of dengue virus entry into HepG2 cells
Cholesterol-lowing effect of taurine in HepG2 cell
Influence of total polar compounds on lipid metabolism, oxidative stress and cytotoxicity in HepG2 cells
Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2
DNMT1/PKR double knockdowned HepG2 (HepG2-DP) cells have high hepatic function and differentiation ability
The Impact of Zinc on Manganese Bioavailability and Cytotoxicity in HepG2 Cells
Involvement of mitochondrial pathway in NCTD-induced cytotoxicity in human hepG2 cells
Artemyrianins A–G from Artemisia myriantha and Their Cytotoxicity Against HepG2 Cells
Comparison of different polar compounds-induced cytotoxicity in human hepatocellular carcinoma HepG2 cells
An in vitro cytotoxicity of glufosfamide in HepG2 cells relative to its nonconjugated counterpart
Synthesis and in vitro cytotoxicity evaluation of isatin-pyrrole derivatives against HepG2 cell line
Pharmakologisch purinerge Modulation des Sauerstoffverbrauchs von HEP-G2 Zellen
Characterization of dengue virus entry into HepG2 cells
Cholesterol-lowing effect of taurine in HepG2 cell
Influence of total polar compounds on lipid metabolism, oxidative stress and cytotoxicity in HepG2 cells
Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2