Cyclization of Short Peptides Designed from Late Embryogenesis Abundant Protein to Improve Stability and Functionality
Abstract: LEA peptides, which are designed based on late embryogenic abundant (LEA) protein sequences, have demonstrated chaperone‐like functions, such as improving drought stress tolerance of Escherichia coli (E. coli). Previous studies have focused on the biological functions of linear LEA peptides. However, the function of cyclic LEA peptide still unknown. This study aimed to explore the cyclic LEA peptides’ bio function like enhance the drought stress tolerance of E. coli by cyclizing the LEA peptide using SICLOPPS (Split Intein Circular Ligation of Peptides and Proteins). The results indicated that cyclization significantly improved the function and extended the potential applications. At the same time, we found that peptides containing numerous lysine residues exhibited reduced performance, which may be due to the exteins’ residues affecting the SICLOPPS efficiency.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Cyclization of Short Peptides Designed from Late Embryogenesis Abundant Protein to Improve Stability and Functionality ; day:20 ; month:02 ; year:2025 ; extent:8
ChemBioChem ; (20.02.2025) (gesamt 8)
- Creator
-
Wu, Yinghan
Ikeno, Shinya
- DOI
-
10.1002/cbic.202401013
- URN
-
urn:nbn:de:101:1-2502211306036.231717156801
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:33 AM CEST
Data provider
This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Wu, Yinghan
- Ikeno, Shinya
Other Objects (12)
LEAPdb: a database for the late embryogenesis abundant proteins
LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana
Abundant expression of somatic transposon-derived piRNAs throughout Tribolium castaneum embryogenesis
Late embryogenesis abundant (LEA) proteins in Arabidopsis thaliana seeds and their role in germination
LEAping to conclusions: A computational reanalysis of late embryogenesis abundant proteins and their possible roles
Alternative conformations of a group 4 Late Embryogenesis Abundant protein associated to its in vitro protective activity
Genome-wide search and structural and functional analyses for late embryogenesis-abundant (LEA) gene family in poplar
Somatic embryogenesis
Torgov Cyclization
Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton
Protein analysis reveals differential accumulation of late embryogenesis abundant and storage proteins in seeds of wild and cultivated amaranth species
Genome-wide analysis and functional validation reveal the role of late embryogenesis abundant genes in strawberry (Fragaria × ananassa) fruit ripening
LEAPdb: a database for the late embryogenesis abundant proteins
LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana
Abundant expression of somatic transposon-derived piRNAs throughout Tribolium castaneum embryogenesis
Late embryogenesis abundant (LEA) proteins in Arabidopsis thaliana seeds and their role in germination
LEAping to conclusions: A computational reanalysis of late embryogenesis abundant proteins and their possible roles
Alternative conformations of a group 4 Late Embryogenesis Abundant protein associated to its in vitro protective activity
Genome-wide search and structural and functional analyses for late embryogenesis-abundant (LEA) gene family in poplar
Somatic embryogenesis
Torgov Cyclization
Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton
Protein analysis reveals differential accumulation of late embryogenesis abundant and storage proteins in seeds of wild and cultivated amaranth species
Genome-wide analysis and functional validation reveal the role of late embryogenesis abundant genes in strawberry (Fragaria × ananassa) fruit ripening
LEAPdb: a database for the late embryogenesis abundant proteins
LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana
Abundant expression of somatic transposon-derived piRNAs throughout Tribolium castaneum embryogenesis
Late embryogenesis abundant (LEA) proteins in Arabidopsis thaliana seeds and their role in germination
LEAping to conclusions: A computational reanalysis of late embryogenesis abundant proteins and their possible roles
Alternative conformations of a group 4 Late Embryogenesis Abundant protein associated to its in vitro protective activity
Genome-wide search and structural and functional analyses for late embryogenesis-abundant (LEA) gene family in poplar
Somatic embryogenesis
Torgov Cyclization
Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton
Protein analysis reveals differential accumulation of late embryogenesis abundant and storage proteins in seeds of wild and cultivated amaranth species