Applicability of Common Methods for Short Time Erosion Analysis In Vitro

Purpose: Dental erosion can be measured by different methods. The aim of the present study was to check the applicability of common methods to determine initial erosive effects. Materials and Methods: Enamel surfaces (4.5 mm2) were eroded in vitro by treatment with hydrochloric acid (pH 2, 2.3 and 2.6) for 5 to 60 s or 240 s, respectively. Erosive effects were assayed with three different methods: Knoop's diamond indentation, profilometry and the determination of the dissolved calcium ions (Ca2+) in a colorimetric assay based on the arsenazo-III-reaction. Results: Erosivemineral loss of > 1 lmaremeasurable with profilometry. This corresponds to the erosive effects that occur after 60 s or more. Profilometric data yielded variance of up to 50%. Knoop's diamond indentation also showed some limitations: the depth of indentation reached a plateau after 30 to 120 s and the measurements showed variance of up to 85%. With the colorimetric assay, short time erosive effects occurring within 5 s could be assessed precisely and kinetically. The method allowed small amounts of 400 pmol Ca2+ per well to be quantified in small volumes with little variability. Conclusions: For evaluation and quantification of short time erosive effects, the colorimetric method is superior to diamond indentation and profilometry.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Applicability of Common Methods for Short Time Erosion Analysis In Vitro ; volume:6 ; number:3 ; year:2008 ; pages:239-248 ; Oral health & preventive dentistry ; 6, Heft 3 (2008), 239-248

Contributors, Places and Time

• Creator: Hannig, Christian ; Becker, Klaus ; Yankeu-Ngalene, Valerie Estelle ; Attin, Thomas

Further information

• DOI: 10.3290/j.ohpd.a13971
• URN: urn:nbn:de:101:1-2023052015075632406254
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 11:00 AM CEST