Poly- l -lysine-caused cell adhesion induces pyroptosis in THP-1 monocytes
Abstract: Pyroptosis is a kind of cell necrosis mediated by inflammasomes. The caspase 1-induced cleavage of gasdermin D (GSDMD) is a canonical pathway to cause membrane pores and eventually cell pyroptosis. Poly-l-lysine (PLL) is widely used to enhance cell adhesion during experiments. Human THP-1 cells are a typical cell line used to study pyroptosis due to their monocytic and macrophage-like characteristics. However, it was found that THP-1 cells seeded on the PLL-coated slides died. To figure out the reason, we observed the morphology of THP-1 cells on PLL-coated slides, which showed obvious pore forming on the cell membranes and cell swelling. The indicated pyroptosis-related protein expression was evaluated and it showed that the conventional caspase-1 pathway of pyroptosis was activated through the NLRP3 inflammasome in THP-1 monocytes on the PLL-coated slides. Hence, PLL-guided cell adhesion induces cell pyroptosis in THP-1 monocytes, which calls for THP-1 dominant studies of pyroptosis to avoid the use of PLL-coated slides or PLL-related drugs.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Poly- l -lysine-caused cell adhesion induces pyroptosis in THP-1 monocytes ; volume:17 ; number:1 ; year:2022 ; pages:279-283 ; extent:5
Open life sciences ; 17, Heft 1 (2022), 279-283 (gesamt 5)
- Urheber
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Yu, Chaoping
Zhao, Wei
Duan, Chujun
Xie, Jiangang
Yin, Wen
- DOI
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10.1515/biol-2022-0028
- URN
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urn:nbn:de:101:1-2022071516480398338001
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:24 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Yu, Chaoping
- Zhao, Wei
- Duan, Chujun
- Xie, Jiangang
- Yin, Wen
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