Statistical optimization of α-amylase production by Bacillus brevis MTCC 7521 in solid-state fermentation using cassava bagasse

Abstract: Production of α-amylase under solid-state fermentation by Bacillus brevis MTCC 7521 has been investigated using cassava bagasse as the substrate, one of the major solid wastes released during extraction of starch from cassava (Manihot esculenta). Response surface methodology was used to evaluate the effect of the main variables, i.e. incubation period (36 h), moisture holding capacity (60%), pH (7.0) and temperature (60°C) on enzyme production by applying a full factorial central composite design. The maximum hydrolysis of soluble starch (85%) and cassava starch (75%) was obtained with the application of 4 mL (≈ 14,752 units) of B. brevis crude enzyme after 5 h of incubation.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
Statistical optimization of α-amylase production by Bacillus brevis MTCC 7521 in solid-state fermentation using cassava bagasse ; volume:64 ; number:5 ; year:2009 ; pages:864-870 ; extent:7
Biologia ; 64, Heft 5 (2009), 864-870 (gesamt 7)

Urheber
Ray, Ramesh
Kar, Shaktimay

DOI
10.2478/s11756-009-0160-1
URN
urn:nbn:de:101:1-2409221631175.510795019077
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:24 MESZ

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Beteiligte

  • Ray, Ramesh
  • Kar, Shaktimay

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