Structure Prediction and Genome Mining‐Aided Discovery of the Bacterial C‐Terminal Tryptophan Prenyltransferase PalQ
Abstract: Post‐translational prenylations, found in eukaryotic primary metabolites and bacterial secondary metabolites, play crucial roles in biomolecular interactions. Employing genome mining methods combined with AlphaFold2‐based predictions of protein interactions, PalQ, a prenyltransferase responsible for the tryptophan prenylation of RiPPs produced by Paenibacillus alvei, is identified. PalQ differs from cyanobactin prenyltransferases because of its evolutionary relationship to isoprene synthases, which enables PalQ to transfer extended prenyl chains to the indole C3 position. This prenylation introduces structural diversity to the tryptophan side chain and also leads to conformational dynamics in the peptide backbone, attributed to the cis/trans isomerization that arises from the formation of a pyrrolidine ring. Additionally, PalQ exhibited pronounced positional selectivity for the C‐terminal tryptophan. Such enzymatic characteristics offer a toolkit for peptide therapeutic lipidation.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Structure Prediction and Genome Mining‐Aided Discovery of the Bacterial C‐Terminal Tryptophan Prenyltransferase PalQ ; day:07 ; month:12 ; year:2023 ; extent:7
Advanced science ; (07.12.2023) (gesamt 7)
- Urheber
-
Miyata, Azusa
Ito, Sohei
Fujinami, Daisuke
- DOI
-
10.1002/advs.202307372
- URN
-
urn:nbn:de:101:1-2023120814374935057018
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:33 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Miyata, Azusa
- Ito, Sohei
- Fujinami, Daisuke
Ähnliche Objekte (12)
Cotranslational folding of HemK C-terminal domain
Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish
Structural Dynamics of the MscL C-terminal Domain
Epigenetic modulation via the C-terminal tail of H2A.Z
Proline isomerization in the C-terminal region of HSP27
Epigenetic modulation via the C-terminal tail of H2A.Z
Elements of the C-terminal tail of a C-terminal domain homolog of the Orange Carotenoid Protein determining xanthophyll uptake from liposomes
Semisynthese biologisch aktiver Insulinanaloga mit C-terminal verkürzter B- Kette
Identification of PKCα as a CK1δ C-terminal targeting kinase
Peptide Cyclization of Unprotected Peptides with Cysteine at C-Terminal
C-Terminal PEGylation Improves SAAP-148 Peptide’s Immunomodulatory Activities
Tissue-specific expression of p73 C-terminal isoforms in mice
Cotranslational folding of HemK C-terminal domain
Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish
Structural Dynamics of the MscL C-terminal Domain
Epigenetic modulation via the C-terminal tail of H2A.Z
Proline isomerization in the C-terminal region of HSP27
Epigenetic modulation via the C-terminal tail of H2A.Z
Elements of the C-terminal tail of a C-terminal domain homolog of the Orange Carotenoid Protein determining xanthophyll uptake from liposomes
Semisynthese biologisch aktiver Insulinanaloga mit C-terminal verkürzter B- Kette
Identification of PKCα as a CK1δ C-terminal targeting kinase
Peptide Cyclization of Unprotected Peptides with Cysteine at C-Terminal
C-Terminal PEGylation Improves SAAP-148 Peptide’s Immunomodulatory Activities
Tissue-specific expression of p73 C-terminal isoforms in mice
Cotranslational folding of HemK C-terminal domain
Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish
Structural Dynamics of the MscL C-terminal Domain
Epigenetic modulation via the C-terminal tail of H2A.Z
Proline isomerization in the C-terminal region of HSP27
Epigenetic modulation via the C-terminal tail of H2A.Z
Elements of the C-terminal tail of a C-terminal domain homolog of the Orange Carotenoid Protein determining xanthophyll uptake from liposomes
Semisynthese biologisch aktiver Insulinanaloga mit C-terminal verkürzter B- Kette
Identification of PKCα as a CK1δ C-terminal targeting kinase
Peptide Cyclization of Unprotected Peptides with Cysteine at C-Terminal
C-Terminal PEGylation Improves SAAP-148 Peptide’s Immunomodulatory Activities