How do the Local Physical, Biochemical, and Mechanical Properties of an Injectable Synthetic Anisotropic Hydrogel Affect Oriented Nerve Growth?
Abstract: As an injectable tissue regenerative platform, Anisogel aims to recapitulate the complex and anisotropic architecture of native extracellular matrix by the use of magneto‐responsive microgels, which are oriented under a low magnetic field of ≈100 mT, while a surrounding hydrogel matrix cross‐links around them. This system promotes the oriented growth of neurons when cultured in vitro. In this study, how the local microgel properties affect neurite outgrowth and orientation is aimed to understand using dorsal root ganglia from chicken embryos. When the surrounding matrix is a synthetic poly (ethylene glycol) hydrogel, the microgel concentration and length required to achieve oriented nerve growth is higher compared to fibrin‐based Anisogels. Microgels should be stiffer than the matrix for cells to sense the mechanical anisotropy but a wide range of microgel stiffness leads to similar cell alignment and growth. On the other hand, modification of the microgels with common extracellular matrix molecules enhances nerve growth but deteriorates nerve alignment compared to bioinert microgels in a cell adhesive surrounding gel. Finally, covalently coupling these microgels to the surrounding matrix reduces both cellular orientation and outgrowth suggesting a reduction in the ability of cells to sense the anisotropy.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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How do the Local Physical, Biochemical, and Mechanical Properties of an Injectable Synthetic Anisotropic Hydrogel Affect Oriented Nerve Growth? ; day:05 ; month:08 ; year:2022 ; extent:15
Advanced functional materials ; (05.08.2022) (gesamt 15)
- Creator
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Babu, Susan
Chen, I.
Vedaraman, Sitara
Gerardo‐Nava, José
Licht, Christopher
Kittel, Yonca
Haraszti, Tamás
Di Russo, Jacopo
De Laporte, Laura
- DOI
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10.1002/adfm.202202468
- URN
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urn:nbn:de:101:1-2022080515183511109282
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:27 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Babu, Susan
- Chen, I.
- Vedaraman, Sitara
- Gerardo‐Nava, José
- Licht, Christopher
- Kittel, Yonca
- Haraszti, Tamás
- Di Russo, Jacopo
- De Laporte, Laura
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How do the local physical, biochemical, and mechanical properties of an injectable synthetic anisotropic hydrogel affect oriented nerve growth?
Digitally Fabricated and Naturally Augmented In Vitro Tissues
Controlling Structure with Injectable Biomaterials to Better Mimic Tissue Heterogeneity and Anisotropy
An Injectable Hybrid Hydrogel with Oriented Short Fibers Induces Unidirectional Growth of Functional Nerve Cells
Nerve Cells Decide to Orient inside an Injectable Hydrogel with Minimal Structural Guidance
Development of in situ assembling microgel-in-hydrogel matrices for directed spinal cord regeneration
A light-modulated hydrogel system to analyze cell durotaxic behavior in a dynamic manner
An Injectable Hybrid Hydrogel with Oriented Short Fibers Induces Unidirectional Growth of Functional Nerve Cells
Controlling Structure with Injectable Biomaterials to Better Mimic Tissue Heterogeneity and Anisotropy
Engineering Anisotropic Cell Models: Development of Collagen Hydrogel Scaffolds with Magneto‐Responsive PEG Microgels for Tissue Engineering Applications
Translating Therapeutic Microgels into Clinical Applications
Digitally Fabricated and Naturally Augmented In Vitro Tissues
How do the local physical, biochemical, and mechanical properties of an injectable synthetic anisotropic hydrogel affect oriented nerve growth?
Digitally Fabricated and Naturally Augmented In Vitro Tissues
Controlling Structure with Injectable Biomaterials to Better Mimic Tissue Heterogeneity and Anisotropy
An Injectable Hybrid Hydrogel with Oriented Short Fibers Induces Unidirectional Growth of Functional Nerve Cells
Nerve Cells Decide to Orient inside an Injectable Hydrogel with Minimal Structural Guidance
Development of in situ assembling microgel-in-hydrogel matrices for directed spinal cord regeneration
A light-modulated hydrogel system to analyze cell durotaxic behavior in a dynamic manner
An Injectable Hybrid Hydrogel with Oriented Short Fibers Induces Unidirectional Growth of Functional Nerve Cells
Controlling Structure with Injectable Biomaterials to Better Mimic Tissue Heterogeneity and Anisotropy
Engineering Anisotropic Cell Models: Development of Collagen Hydrogel Scaffolds with Magneto‐Responsive PEG Microgels for Tissue Engineering Applications
Translating Therapeutic Microgels into Clinical Applications