An Enabling Peptide Ligation Induced by Thiol‐Salicylaldehyde Ester for Chemical Protein Synthesis
Abstract: Chemical protein synthesis by amide‐forming ligation of two unprotected peptide segments offers an effective strategy for the preparation of protein derivatives that are not accessible through bioengineering approaches. Herein, an unprecedented chemical ligation between peptides with C‐terminal 2‐mercaptobenzaldehyde (thiol‐salicylaldehyde, TSAL) esters and peptides bearing N‐terminal cysteine/penicillamine is reported. Reactive peptide TSAL esters can be obtained from peptide hydrazides in an operationally simple and highly effective manner. This chemoselective peptide ligation enables the rapid production of N,S‐benzylidene acetal intermediates, which can readily be converted into native amide bonds even at sterically hindered junctions. In addition, the current method can be applied compatibly in concert with other types of ligations and subsequent desulfurization chemistry, thereby facilitating convergent protein synthesis. The effectiveness of this new method is also showcased by the total synthesis of proteins ubiquitin and hyalomin‐3 (Hyal‐3), the efficient synthesis of protein ubiquitin‐fold modifier 1 (UFM1) via a C‐to‐N sequential TSAL ester‐induced ligation strategy, and the chemical synthesis of protein Mtb CM through a combined strategy of Ser/Thr ligation and TSAL ester‐induced ligations.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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An Enabling Peptide Ligation Induced by Thiol‐Salicylaldehyde Ester for Chemical Protein Synthesis ; day:23 ; month:10 ; year:2024 ; extent:10
Advanced science ; (23.10.2024) (gesamt 10)
- Creator
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Li, Cuicui
Ma, Wenge
Jin, Kang
- DOI
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10.1002/advs.202408538
- URN
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urn:nbn:de:101:1-2410231449565.775402115436
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:26 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Li, Cuicui
- Ma, Wenge
- Jin, Kang
Other Objects (12)
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Peptide‐Hypervalent Iodine Reagent Chimeras: Enabling Peptide Functionalization and Macrocyclization **
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Biodegradability of unsaturated poly(ester-thioether)s synthesized by thiol-yne reactions
Thiol-Labile Amino Protecting Group that Suppresses the Epimerization in Peptide Synthesis
HF‐Free Boc Synthesis of Peptide Thioesters for Ligation and Cyclization
HF‐Free Boc Synthesis of Peptide Thioesters for Ligation and Cyclization
Cyclic Peptide Synthesis Using Peptidyl Salicylaldehyde Esters
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Sulfide-Mediated Peptide Ligation in Water
Development of a tert-butyl thiol linker for the synthesis of peptide thioesters and thioacids for application in chemical ligation methods
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Peptide‐Hypervalent Iodine Reagent Chimeras: Enabling Peptide Functionalization and Macrocyclization **
Peptide‐Hypervalent Iodine Reagent Chimeras: Enabling Peptide Functionalization and Macrocyclization **
Biodegradability of unsaturated poly(ester-thioether)s synthesized by thiol-yne reactions
Thiol-Labile Amino Protecting Group that Suppresses the Epimerization in Peptide Synthesis
HF‐Free Boc Synthesis of Peptide Thioesters for Ligation and Cyclization
HF‐Free Boc Synthesis of Peptide Thioesters for Ligation and Cyclization