The Role of Tricellulin in Epithelial Jamming and Unjamming via Segmentation of Tricellular Junctions

Abstract: Collective cellular behavior in confluent monolayers supports physiological and pathological processes of epithelial development, regeneration, and carcinogenesis. Here, the attainment of a mature and static tissue configuration or the local reactivation of cell motility involve a dynamic regulation of the junctions established between neighboring cells. Tricellular junctions (tTJs), established at vertexes where three cells meet, are ideally located to control cellular shape and coordinate multicellular movements. However, their function in epithelial tissue dynamic remains poorly defined. To investigate the role of tTJs establishment and maturation in the jamming and unjamming transitions of epithelial monolayers, a semi‐automatic image‐processing pipeline is developed and validated enabling the unbiased and spatially resolved determination of the tTJ maturity state based on the localization of fluorescent reporters. The software resolves the variation of tTJ maturity accompanying collective transitions during tissue maturation, wound healing, and upon the adaptation to osmolarity changes. Altogether, this work establishes junctional maturity at tricellular contacts as a novel biological descriptor of collective responses in epithelial monolayers.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: The Role of Tricellulin in Epithelial Jamming and Unjamming via Segmentation of Tricellular Junctions ; volume:7 ; number:15 ; year:2020 ; extent:13 ; Advanced science ; 7, Heft 15 (2020) (gesamt 13)

Beteiligte, Orts- und Zeitangaben

• Urheber: Lohmann, Sophie ; Giampietro, Costanza ; Pramotton, Francesca M. ; Al‐Nuaimi, Dunja ; Poli, Alessandro ; Maiuri, Paolo ; Poulikakos, Dimos ; Ferrari, Aldo

Weitere Informationen

• DOI: 10.1002/advs.202001213
• URN: urn:nbn:de:101:1-2022062707272486953122
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:26 MESZ