The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans
Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA–Argonaute complex—or miRNA‐induced silencing complex (miRISC)—can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C‐kinase (RACK1), is important for miRNA‐mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post‐initiation mode of miRNA‐mediated gene repression.
RACK1, a constituent of the ribosomal 40S subunit, is required for the association of miRISC with translating ribosomes. This suggests that RACK1 contributes to recruit miRISC to the site of translation and supports a post‐initiation mode of miRNA‐mediated gene repression.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans ; volume:12 ; number:6 ; year:2011 ; pages:581-586 ; extent:6
EMBO reports / European Molecular Biology Organization ; 12, Heft 6 (2011), 581-586 (gesamt 6)
- Creator
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Jannot, Guillaume
Bajan, Sarah
Giguère, Nellie J.
Bouasker, Samir
Banville, Isabelle H.
Piquet, Sandra
Hutvagner, Gyorgy
Simard, Martin J.
- DOI
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10.1038/embor.2011.66
- URN
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urn:nbn:de:101:1-2023031707471765392971
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:45 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Jannot, Guillaume
- Bajan, Sarah
- Giguère, Nellie J.
- Bouasker, Samir
- Banville, Isabelle H.
- Piquet, Sandra
- Hutvagner, Gyorgy
- Simard, Martin J.
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Functional characterization of Pol5 in the maturation of both ribosomal subunits
Ribosomal protein L24 mediates mammalian microRNA processing in an evolutionarily conserved manner
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C. elegans dss-1is functionally conserved and required for oogenesis and larval growth
TRPM channels are required for rhythmicity in the ultradian defecation rhythm of C. elegans
Regulatory elements of Caenorhabditis elegans ribosomal protein genes
Both the apoptotic suicide pathway and phagocytosis are required for a programmed cell death in Caenorhabditis elegans
The ribosomal protein P0A is required for embryo development in rice
Functional characterization of Pol5 in the maturation of both ribosomal subunits
Ribosomal protein L24 mediates mammalian microRNA processing in an evolutionarily conserved manner
The roles of ribosomal proteins in nasopharyngeal cancer: culprits, sentinels or both
Lower expression of genes near microRNA in C. elegans germline
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Quantification of microRNA in plasma using probe based TaqMan assays: is microRNA purification required?
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