Short-term biological variation of serum tryptase

Objectives: Serum tryptase is a biomarker of mast cell activation. Among others, it is used in the diagnosis of anaphylaxis where a significant increase during the acute phase supports the diagnosis. When evaluating changes in biomarker levels, it is of utmost importance to consider the biological variation of the marker. Therefore, the aim of this study was to evaluate the short-term biological variation of serum tryptase. Methods: Blood samples were drawn at 9 AM three days in a row from apparently healthy subjects. On day two, additional blood samples were drawn every third hour for 12 h. The tryptase concentration was measured in serum using a fluoroenzyme immunoassay (ImmunoCAP™, Thermo Fisher Scientific). Linear mixed-effects models were used to calculate components of biological variation. Results: In 32 subjects, the overall mean concentration of tryptase was 4.0 ng/mL (range, 1.3–8.0 ng/mL). The within-subject variation was 3.7 % (95 % confidence interval (CI) 3.0–4.4 %), the between-subject variation was 31.5 % (95 % CI 23.1–39.8 %), and the analytical variation was 3.4 % (95 % CI 2.9–4.1 %). The reference change value was 13.3 % for an increase in tryptase at a 95 % level of significance. No significant day-to-day variation was observed (p=0.77), while a minute decrease in the serum concentration was observed during the day (p<0.0001). Conclusions: Serum tryptase is a tightly regulated biomarker with very low within-subject variation, no significant day-to-day variation, and only minor semidiurnal variation. In contrast, a considerable between-subject variation exists. This establishes serum tryptase as a well-suited biomarker for monitoring.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Short-term biological variation of serum tryptase ; volume:62 ; number:4 ; year:2024 ; pages:713-719 ; extent:07 ; Clinical chemistry and laboratory medicine ; 62, Heft 4 (2024), 713-719 (gesamt 07)

Beteiligte, Orts- und Zeitangaben

• Urheber: Madsen, Anne Tranberg ; Kristiansen, Helle Pilgaard ; Winther-Larsen, Anne

Weitere Informationen

• DOI: 10.1515/cclm-2023-0606
• URN: urn:nbn:de:101:1-2024022713084208606740
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 14.08.2025, 10:57 MESZ