Through bonds or contacts? Mapping protein vibrational energy transfer using non-canonical amino acids
Abstract: Vibrational energy transfer (VET) is essential for protein function. It is responsible for efficient energy dissipation in reaction sites, and has been linked to pathways of allosteric communication. While it is understood that VET occurs via backbone as well as via non-covalent contacts, little is known about the competition of these two transport channels, which determines the VET pathways. To tackle this problem, we equipped the β-hairpin fold of a tryptophan zipper with pairs of non-canonical amino acids, one serving as a VET injector and one as a VET sensor in a femtosecond pump probe experiment. Accompanying extensive non-equilibrium molecular dynamics simulations combined with a master equation analysis unravel the VET pathways. Our joint experimental/computational endeavor reveals the efficiency of backbone vs. contact transport, showing that even if cutting short backbone stretches of only 3 to 4 amino acids in a protein, hydrogen bonds are the dominant VET pathway
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Nature communications. - 12 (2021) , 3284, ISSN: 2041-1723
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2021
- Creator
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Deniz, Erhan
Valiño Borau, Luis
Löffler, Jan G.
Eberl, Katharina Barbara
Gulzar, Adnan
Wolf, Steffen
Durkin, Patrick M.
Kaml, Robert
Budisa, Nediljko
Stock, Gerhard
Bredenbeck, Jens
- DOI
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10.1038/s41467-021-23591-1
- URN
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urn:nbn:de:bsz:25-freidok-2182367
- Rights
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Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:49 PM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Deniz, Erhan
- Valiño Borau, Luis
- Löffler, Jan G.
- Eberl, Katharina Barbara
- Gulzar, Adnan
- Wolf, Steffen
- Durkin, Patrick M.
- Kaml, Robert
- Budisa, Nediljko
- Stock, Gerhard
- Bredenbeck, Jens
- Universität
Time of origin
- 2021
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Relations between canonical and non-canonical inflation
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Engineering of lanthipeptides using standard canonical amino acids and noncanonical amino acids
Non-Canonical Grammar!?
Applying non-canonical amino acids for investigation of vibrational energy transfer and dynamic allostery in a synaptic protein domain
Non-canonical lassen-middles
Non-Canonical Amino Acids in Analyses of Protease Structure and Function
Enzymatic Production Of Chiral Non-Canonical Amino Acids With Decarbamoylating Enzymes
Relations between canonical and non-canonical inflation
Design and generation of antibody-drug conjugates using non-canonical amino acids
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