Co-culture of Helicobacter pylori with oral microorganisms in human saliva

zu Verbundenen Objekten

Abstract: Objective
Helicobacter pylori is known for colonizing the gastric mucosa and instigating severe upper gastrointestinal diseases such as gastritis, gastroduodenal ulcers, and gastric cancer. To date, there is no data available on the oral cavity as transmission site, whether H. pylori can survive in the oral cavity or in human saliva. The aim of the study was to investigate the influence of oral microorganisms and human saliva on the survival of H. pylori in human saliva.

Methods
H. pylori strains KE, a motile derivate of type strain H. pylori 26695, and H. pylori SS1, a clinical isolate from a gastric biopsy, were grown in human pooled saliva (pooled from 4 healthy human donors, 0.22 μm filter-sterilized) or in BBF (Brucella browth formula; control) either as mono-cultures or in co-culture with Streptococcus mutans, Streptococcus oralis, Actinomyces naeslundii, Lacticaseibacillus casei and Candida dubliniensis. Bacterial survival of H. pylori and the oral microorganisms were investigated using colony forming units (CFU) assay and MALDI-TOF MS at baseline and after 24, 48 and 168 h.

Results
In saliva, H. pylori KE demonstrated enhanced survival in co-culture with S. mutans, A. naeslundii, and C. dubliniensis, enduring for at least 48 h. In contrast, L. casei and S. oralis inhibited H. pylori KE in saliva. H. pylori KE could not be cultured after 168 h in saliva, neither in mono- nor co-culture. In contrast, H. pylori SS1 in saliva could be cultured after 168 h in co-culture with S. mutans and C. dubliniensis, but not in mono-culture. In BBF, H. pylori KE could be cultured after 168 h with S. mutans, L. casei and C. dubliniensis, and H. pylori SS1 with L. casei and C. dubliniensis, but not with S. mutans. Notably, the co-cultured microorganisms survived at high CFU numbers similar to those of the monocultures.

Conclusion
The study suggests that H. pylori can transiently survive in human saliva and even with presence of certain oral microorganisms. However, it may not be a permanent resident of the oral microbiota. The co-survival with oral microorganisms emphasizes the necessity for studying the role of the oral microbiota in the infectious and transmission cycle of H. pylori

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch
Anmerkungen
Clinical oral investigations. - 29, 1 (2025) , 79, ISSN: 1436-3771

Ereignis
Veröffentlichung
(wo)
Freiburg
(wer)
Universität
(wann)
2025
Urheber

DOI
10.1007/s00784-025-06160-4
URN
urn:nbn:de:bsz:25-freidok-2627282
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:20 MESZ

Datenpartner

Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Original beim Datenpartner anzeigen

Beteiligte

Entstanden

  • 2025

Ähnliche Objekte (12)

Antimicrobial photodynamic treatment with mother juices and their single compounds as photosensitizers

Antimicrobial photodynamic treatment with mother juices and their single compounds as photosensitizers

Natural antimicrobials and oral microorganisms: a systematic review on herbal interventions for the eradication of multispecies oral biofilms

Natural antimicrobials and oral microorganisms: a systematic review on herbal interventions for the eradication of multispecies oral biofilms

Fluoridverteilung zwischen Plaque, gesundem und kariösem Zahnschmelz nach lokaler Anwendung von drei gebräuchlichen Fluoriden : e. In-vitro-Unters.

Hochschulschrift

Fluoridverteilung zwischen Plaque, gesundem und kariösem Zahnschmelz nach lokaler Anwendung von drei gebräuchlichen Fluoriden : e. In-vitro-Unters.

Untersuchungen zur biologischen Abbaubarkeit und Bakterientoxizität klinikspezifischer Abwasserinhaltsstoffe und deren Einfluß auf die Populationsdynamik im Belebtschlamm

Hochschulschrift

Untersuchungen zur biologischen Abbaubarkeit und Bakterientoxizität klinikspezifischer Abwasserinhaltsstoffe und deren Einfluß auf die Populationsdynamik im Belebtschlamm

In vitro eradication of planktonic, saliva and biofilm bacteria using lingonberry extract as a photosensitizer for visible light plus water-filtered infrared-A irradiation

In vitro eradication of planktonic, saliva and biofilm bacteria using lingonberry extract as a photosensitizer for visible light plus water-filtered infrared-A irradiation

Does human saliva decrease the antimicrobial activity of chlorhexidine against oral bacteria?

Does human saliva decrease the antimicrobial activity of chlorhexidine against oral bacteria?

Microbial composition of oral biofilms after visible light and water-filtered infrared a radiation (VIS+wIRA) in combination with Indocyanine Green (ICG) as photosensitizer

Microbial composition of oral biofilms after visible light and water-filtered infrared a radiation (VIS+wIRA) in combination with Indocyanine Green (ICG) as photosensitizer

Influence of probiotics on the salivary microflora oral streptococci and their integration into oral biofilm

Influence of probiotics on the salivary microflora oral streptococci and their integration into oral biofilm

Antimicrobial behavior and cytotoxicity of indocyanine green in combination with visible light and water-filtered infrared a radiation against periodontal bacteria and subgingival biofilm

Antimicrobial behavior and cytotoxicity of indocyanine green in combination with visible light and water-filtered infrared a radiation against periodontal bacteria and subgingival biofilm

Resistance toward chlorhexidine in oral bacteria - is there cause for concern?

Resistance toward chlorhexidine in oral bacteria - is there cause for concern?

Influence of the long-term use of oral hygiene products containing stannous ions on the salivary microbiome – a randomized controlled trial

Influence of the long-term use of oral hygiene products containing stannous ions on the salivary microbiome – a randomized controlled trial

Antimicrobial photoinactivation of in situ oral biofilms by visible light plus water-filtered infrared A and tetrahydroporphyrin-tetratosylate (THPTS)

Antimicrobial photoinactivation of in situ oral biofilms by visible light plus water-filtered infrared A and tetrahydroporphyrin-tetratosylate (THPTS)

Verbundene Objekte