Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon
Abstract: Bacterial membrane vesicles have emerged as gadgets allowing remote communication between the microbiota and distal host organs. Here we describe a protocol for enriching vesicles from serum and colon that could widely be adapted for other tissues. We detail pre-clearing of serum or colon fluids using 0.2-μm syringe filters and their concentration by centrifugal filter devices. We also describe vesicle isolation with qEV size exclusion columns and finally the concentration of isolated vesicle fractions for downstream analyses
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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STAR protocols. - 4, 1 (2023) , 102046, ISSN: 2666-1667
- Ereignis
-
Veröffentlichung
- (wo)
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Freiburg
- (wer)
-
Universität
- (wann)
-
2023
- Urheber
- DOI
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10.1016/j.xpro.2023.102046
- URN
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urn:nbn:de:bsz:25-freidok-2343923
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
25.03.2025, 13:49 MEZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
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Beteiligte
- Erttmann, Saskia Friederike
- Gekara, Nelson O.
- Universität
Entstanden
- 2023
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