Simple Protocol for immunoglobulin G Purification from Camel “ Camelus dromedarius ” Serum
Abstract: The present study aimed to describe and standardize a simple and efficient protocol for purification of camel IgG from serum, which can be applied for Camilidae antibody production in research laboratories, the preindustrial stage. Camel serum IgG was separated with caprylic acid and ammonium sulfate, then the effect of four variables studied: caprylic acid concentration, pH, stirring time, and stirring intensity. Camel IgG prepared by standardized caprylic acid fractionation method for camel serum was compared with commercial anti-sera products. Camel IgG purification from undiluted sera using caprylic acid at concentration of 8% v/v gave the best results. Purification at different pH values using caprylic acid at 8% v/v revealed that pH 5.5 was optimal. Investigating purification at different stirring time intervals using 8% v/v caprylic acid at pH 5.5 demonstrated that stirring for 90 min gave the optimum results. Finally, studying purification at different stirring intensities using 8% v/v caprylic acid at pH 5.5 for 90 min, the best stirring intensity was at 450 rpm. Overall, the results suggest that caprylic acid purification of camel serum IgG is more effective and safe than ammonium sulfate method in simplicity, purity, and lower non-IgG proteins in the final preparation with lower protein aggregates.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Simple Protocol for immunoglobulin G Purification from Camel “ Camelus dromedarius ” Serum ; volume:12 ; number:1 ; year:2017 ; pages:143-155 ; extent:13
Open life sciences ; 12, Heft 1 (2017), 143-155 (gesamt 13)
- Creator
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Shawki, Asmaa
Abd El-Baky, Nawal
Ahmed, Mohammed
Linjawi, Mustafa H.
Aljaddawi, Abdullah A.
Redwan, Elrashdy M.
- DOI
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10.1515/biol-2017-0017
- URN
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urn:nbn:de:101:1-2409201638514.970878931566
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:20 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Shawki, Asmaa
- Abd El-Baky, Nawal
- Ahmed, Mohammed
- Linjawi, Mustafa H.
- Aljaddawi, Abdullah A.
- Redwan, Elrashdy M.
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Genetic parameters of birth weight trait in dromedary camels (Camelus dromedarius)
Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa
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Tuberculosis caused by Mycobacterium caprae in a camel (Camelus dromedarius)
Genetic parameters of birth weight trait in dromedary camels (Camelus dromedarius)
Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa
Echocardiography of the normal camel (Camelus dromedaries) heart: technique and cardiac dimensions
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