A simple and effective method for protein subcellular localization using Agrobacterium-mediated transformation of onion epidermal cells
Abstract: A modified Agrobacterium-mediated transformation protocol has been successfully used for transient expression of the intrinsically fluorescent proteins and their fusion proteins in onion epidermis. The mean of the transformed cells rate per peel is about 10.5±0.9%, while that of the particle bombardment method is at the range 2.0±0.4%. To compare with the prevailing method of micro-projectile bombardment, the modified Agrobacterium-mediated transformation may provide with higher efficiency and even more simplified manipulability on a lower budget.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
A simple and effective method for protein subcellular localization using Agrobacterium-mediated transformation of onion epidermal cells ; volume:62 ; number:5 ; year:2007 ; pages:529-532 ; extent:4
Biologia ; 62, Heft 5 (2007), 529-532 (gesamt 4)
- Urheber
-
Sun, Wei
Cao, Ziyi
Li, Yan
Zhao, Yanxiu
Zhang, Hui
- DOI
-
10.2478/s11756-007-0104-6
- URN
-
urn:nbn:de:101:1-2409221547239.683701170071
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:28 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Sun, Wei
- Cao, Ziyi
- Li, Yan
- Zhao, Yanxiu
- Zhang, Hui
Ähnliche Objekte (12)
The productivity of return migrants: The case of China's Sea Turtles
Using sums-of-squares to prove Gaussian product inequalities
RNA isoform analyses of Drosophila Dscam gene and Xenopus tropicalis clustered Protocadherin genes provide insights for neuronal self-avoidance
Biomimetic design and fabrication of tissue scaffolds : using computer aided engineering
Plague vaccine: recent progress and prospects
A unified stochastic SIR model driven by Lévy noise with time-dependency
Stanford type A acute aortic dissection with proximal intimo-intimal intussusception: a case report and literature review
A holographic matrix representation of the metamorphic parallel mechanisms
CAR-T cell therapy shines as a beam of hope in the fight against acute leukemia
Small Two-Bar Specimen Creep Testing of Grade P91 Steel at 650°C
The causal effects of childhood sunburn occasions on melanoma: A univariable and multivariable Mendelian randomization study
Saccharide analysis of onion outer epidermal walls
The productivity of return migrants: The case of China's Sea Turtles
Using sums-of-squares to prove Gaussian product inequalities
RNA isoform analyses of Drosophila Dscam gene and Xenopus tropicalis clustered Protocadherin genes provide insights for neuronal self-avoidance
Biomimetic design and fabrication of tissue scaffolds : using computer aided engineering
Plague vaccine: recent progress and prospects
A unified stochastic SIR model driven by Lévy noise with time-dependency
Stanford type A acute aortic dissection with proximal intimo-intimal intussusception: a case report and literature review
A holographic matrix representation of the metamorphic parallel mechanisms
CAR-T cell therapy shines as a beam of hope in the fight against acute leukemia
Small Two-Bar Specimen Creep Testing of Grade P91 Steel at 650°C
The causal effects of childhood sunburn occasions on melanoma: A univariable and multivariable Mendelian randomization study
Saccharide analysis of onion outer epidermal walls
The productivity of return migrants: The case of China's Sea Turtles
Using sums-of-squares to prove Gaussian product inequalities
RNA isoform analyses of Drosophila Dscam gene and Xenopus tropicalis clustered Protocadherin genes provide insights for neuronal self-avoidance
Biomimetic design and fabrication of tissue scaffolds : using computer aided engineering
Plague vaccine: recent progress and prospects
A unified stochastic SIR model driven by Lévy noise with time-dependency
Stanford type A acute aortic dissection with proximal intimo-intimal intussusception: a case report and literature review
A holographic matrix representation of the metamorphic parallel mechanisms
CAR-T cell therapy shines as a beam of hope in the fight against acute leukemia
Small Two-Bar Specimen Creep Testing of Grade P91 Steel at 650°C
The causal effects of childhood sunburn occasions on melanoma: A univariable and multivariable Mendelian randomization study