Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single‐cell profiling

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Abstract: Similar to the brain, the eye is considered an immune‐privileged organ where tissue‐resident macrophages provide the major immune cell constituents. However, little is known about spatially restricted macrophage subsets within different eye compartments with regard to their origin, function, and fate during health and disease. Here, we combined single‐cell analysis, fate mapping, parabiosis, and computational modeling to comprehensively examine myeloid subsets in distinct parts of the eye during homeostasis. This approach allowed us to identify myeloid subsets displaying diverse transcriptional states. During choroidal neovascularization, a typical hallmark of neovascular age‐related macular degeneration (AMD), we recognized disease‐specific macrophage subpopulations with distinct molecular signatures. Our results highlight the heterogeneity of myeloid subsets and their dynamics in the eye that provide new insights into the innate immune system in this organ which may offer new therapeutic targets for ophthalmological diseases

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Notes: The EMBO Journal. - (2021) , e105123, ISSN: 1460-2075

Event: Veröffentlichung

(where): Freiburg

(who): Universität

(when): 2021

Creator: Wieghofer, Peter
Hagemeyer, Nora
Sankowski, Roman
Schlecht, Anja
Staszewski, Ori
Amann, Lukas
Gruber, Markus
Koch, Jana
Hausmann, Annika
Zhang, Pei Pei
Boneva, Stefaniya
Masuda, Takahiro
Hilgendorf, Ingo
Goldmann, Tobias
Böttcher, Chotima
Priller, Josef
Rossi, Fabio M. V.
Lange, Clemens
Prinz, Marco

DOI: 10.15252/embj.2020105123

URN: urn:nbn:de:bsz:25-freidok-1938241

Rights: Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 25.03.2025, 1:46 PM CET

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2021

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