Exploring the Substrate Scope and Catalytic Promiscuity of Nitroreductase‐Like Enzymes

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Abstract: Flavin‐dependent nitroreductases are gaining attention as biocatalysts for the synthesis of pharmaceutically active compounds and their precursors. Here, we examined a panel of nitroreductase‐like flavoenzymes for their reductase activity towards a wide variety of aromatic and aliphatic nitro compounds, nitroolefins, and α,β‐unsaturated carbonyl compounds. Several of these flavoenzymes displayed high reductase activity and achieved excellent conversion of diverse nitroarenes, nitroolefins and α,β‐unsaturated carbonyl compounds, accomplishing good product yields in semi‐preparative scale reactions (up to 97%). In addition to the catalytic promiscuity of several of these flavoenzymes, being able to perform the reduction of nitro groups (nitroreductase activity) as well as C=C groups (ene‐reductase activity), this study also revealed that some flavoenzymes exhibit high chemo‐, regio‐ and/or enantioselectivity, making them attractive enzymes for use in organic synthesis.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Exploring the Substrate Scope and Catalytic Promiscuity of Nitroreductase‐Like Enzymes ; day:21 ; month:10 ; year:2024 ; extent:10
Advanced synthesis & catalysis ; (21.10.2024) (gesamt 10)

Creator: Luján, Alejandro Prats
Bhat, Mohammad Faizan
Saravanan, Thangavelu
Poelarends, Gerrit

DOI: 10.1002/adsc.202400220

URN: urn:nbn:de:101:1-2410221434128.891128029570

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:29 AM CEST

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Luján, Alejandro Prats
Bhat, Mohammad Faizan
Saravanan, Thangavelu
Poelarends, Gerrit

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Exploring the Substrate Scope and Catalytic Promiscuity of Nitroreductase‐Like Enzymes

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