Improved phosphate metabolism and biomass production by overexpression of AtPAP18 in tobacco
Abstract: Limited availability of phosphate ion (Pi) reduces plant growth in natural ecosystems. Here, we report the functional effects of overexpressing an Arabidopsis thaliana purple acid phosphatase encoding gene, AtPAP18, in Nicotiana tabbacum as a crop model plant. Transgenic tobacco plants exhibited significant increases in acid phosphatase activity, total P and Pi contents leading to improved biomass production in both Pi-deficient and Pi-sufficient conditions. Transient expression of AtPAP18::green fluorescent fusion protein in onion epidermal cells indicated that AtPAP18 is a dual-targeted protein, which is detected mainly in the apoplast of the cells after 24 h and in the vacuole after 72 h. Possibly, AtPAP18 protein confers efficient retrieval of Pi from bonded extracellular compounds as well as expendable intracellular Pi-monoesters and anhydrides. These data clearly indicate that overexpression of AtPAP18 gene offers an effective approach for reducing the consumption of chemical Pi fertilizer through increased acquisition of soil Pi and mobilization of internal resources.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Improved phosphate metabolism and biomass production by overexpression of AtPAP18 in tobacco ; volume:67 ; number:4 ; year:2012 ; pages:713-720 ; extent:8
Biologia ; 67, Heft 4 (2012), 713-720 (gesamt 8)
- Creator
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Zamani, Katayoun
Sabet, Mohammad
Lohrasebi, Tahmineh
Mousavi, Amir
Malboobi, Mohammad
- DOI
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10.2478/s11756-012-0072-3
- URN
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urn:nbn:de:101:1-2409221714034.562684461427
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:29 AM CEST
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Associated
- Zamani, Katayoun
- Sabet, Mohammad
- Lohrasebi, Tahmineh
- Mousavi, Amir
- Malboobi, Mohammad
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Jak1/Stat3 Activation Alters Phosphate Metabolism Independently of Sex and Extracellular Phosphate Levels
Hepatic Acat2 overexpression promotes systemic cholesterol metabolism and adipose lipid metabolism in mice
Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco
Environmental tobacco smoke exposure exaggerates bleomycin-induced collagen overexpression during pulmonary fibrogenesis
Integrated Furnace for Combustion/Gasification of Biomass Fuel for Tobacco Curing
Overexpression of DnWRKY11 enhanced salt and drought stress tolerance of transgenic tobacco
Tobacco induces abnormal metabolism of tryptophan via the kynurenine pathway
Impact of leptin or melatonin on Sema4D overexpression-related bone metabolism
Heterologous mannitol-1-phosphate dehydrogenase gene over-expression in Parachlorella kessleri for enhanced microalgal biomass productivity
Overexpression of pyruvate, orthophosphate dikinase facilitates phosphate uptake and allows better growth of tobacco in alkaline soil
Overexpression of Pyruvate, Orthophosphate Dikinase Facilitates Phosphate Uptake and Allows Better Growth of Tobacco in Alkaline Soil
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Jak1/Stat3 Activation Alters Phosphate Metabolism Independently of Sex and Extracellular Phosphate Levels
Hepatic Acat2 overexpression promotes systemic cholesterol metabolism and adipose lipid metabolism in mice
Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco
Environmental tobacco smoke exposure exaggerates bleomycin-induced collagen overexpression during pulmonary fibrogenesis
Integrated Furnace for Combustion/Gasification of Biomass Fuel for Tobacco Curing
Overexpression of DnWRKY11 enhanced salt and drought stress tolerance of transgenic tobacco
Tobacco induces abnormal metabolism of tryptophan via the kynurenine pathway