PGC-1α is associated with C2C12 Myoblast differentiation

Abstract: PGC-1α has been implicated as an important mediator of functional capacity of skeletal muscle. However, the role of PGC-1α in myoblast differentiation remains unexplored. In the present study, we observed a significant up-regulation of PGC-1α expression during the differentiation of murine C2C12 myoblast. To understand the biological significance of PGC-1α up-regulation in myoblast differentiation, C2C12 cells were transfected with murine PGC-1α cDNA and siRNA targeting PGC-1α, respectively. PGC-1α over-expressing clones fused to form typical myotubes with higher mRNA level of myosin heavy chain isoform I (MyHCI) and lower MyHCIIX. No obvious differentiation was observed in PGC-1α-targeted siRNA-transfected cells with marked decrement of mRNA levels of MyHCI and MyHCIIX. Furthermore, PGC-1α increased the expression of MyoD and MyoG in C2C12 cells, which controlled the commitment of precursor cells to myotubes. These results indicate that PGC-1α is associated with myoblast differentiation and elevates MyoD and MyoG expression levels in C2C12 cells.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: PGC-1α is associated with C2C12 Myoblast differentiation ; volume:9 ; number:11 ; year:2014 ; pages:1030-1036 ; extent:7 ; Open life sciences ; 9, Heft 11 (2014), 1030-1036 (gesamt 7)

Contributors, Places and Time

• Creator: Lin, Yaqiu ; Zhao, Yanying ; Li, Ruiwen ; Gong, Jiaqi ; Zheng, Yucai ; Wang, Yong

Further information

• DOI: 10.2478/s11535-014-0341-y
• URN: urn:nbn:de:101:1-2409211613529.293409871230
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:22 AM CEST