Alternative splicing coupled mRNA decay shapes the temperature‐dependent transcriptome
Abstract: Mammalian body temperature oscillates with the time of the day and is altered in diverse pathological conditions. We recently identified a body temperature‐sensitive thermometer‐like kinase, which alters SR protein phosphorylation and thereby globally controls alternative splicing (AS). AS can generate unproductive variants which are recognized and degraded by diverse mRNA decay pathways—including nonsense‐mediated decay (NMD). Here we show extensive coupling of body temperature‐controlled AS to mRNA decay, leading to global control of temperature‐dependent gene expression (GE). Temperature‐controlled, decay‐inducing splicing events are evolutionarily conserved and pervasively found within RNA‐binding proteins, including most SR proteins. AS‐coupled poison exon inclusion is essential for rhythmic GE of SR proteins and has a global role in establishing temperature‐dependent rhythmic GE profiles, both in mammals under circadian body temperature cycles and in plants in response to ambient temperature changes. Together, these data identify body temperature‐driven AS‐coupled mRNA decay as an evolutionary ancient, core clock‐independent mechanism to generate rhythmic GE.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Alternative splicing coupled mRNA decay shapes the temperature‐dependent transcriptome ; volume:21 ; number:12 ; year:2020 ; extent:17
EMBO reports / European Molecular Biology Organization ; 21, Heft 12 (2020) (gesamt 17)
- Urheber
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Neumann, Alexander
Meinke, Stefan
Goldammer, Gesine
Strauch, Miriam
Schubert, Daniel
Timmermann, Bernd
Heyd, Florian
Preußner, Marco
- DOI
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10.15252/embr.202051369
- URN
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urn:nbn:de:101:1-2022070408083254364741
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:28 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Neumann, Alexander
- Meinke, Stefan
- Goldammer, Gesine
- Strauch, Miriam
- Schubert, Daniel
- Timmermann, Bernd
- Heyd, Florian
- Preußner, Marco
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