Alternative splicing coupled mRNA decay shapes the temperature‐dependent transcriptome
Abstract: Mammalian body temperature oscillates with the time of the day and is altered in diverse pathological conditions. We recently identified a body temperature‐sensitive thermometer‐like kinase, which alters SR protein phosphorylation and thereby globally controls alternative splicing (AS). AS can generate unproductive variants which are recognized and degraded by diverse mRNA decay pathways—including nonsense‐mediated decay (NMD). Here we show extensive coupling of body temperature‐controlled AS to mRNA decay, leading to global control of temperature‐dependent gene expression (GE). Temperature‐controlled, decay‐inducing splicing events are evolutionarily conserved and pervasively found within RNA‐binding proteins, including most SR proteins. AS‐coupled poison exon inclusion is essential for rhythmic GE of SR proteins and has a global role in establishing temperature‐dependent rhythmic GE profiles, both in mammals under circadian body temperature cycles and in plants in response to ambient temperature changes. Together, these data identify body temperature‐driven AS‐coupled mRNA decay as an evolutionary ancient, core clock‐independent mechanism to generate rhythmic GE.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Alternative splicing coupled mRNA decay shapes the temperature‐dependent transcriptome ; volume:21 ; number:12 ; year:2020 ; extent:17
EMBO reports / European Molecular Biology Organization ; 21, Heft 12 (2020) (gesamt 17)
- Creator
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Neumann, Alexander
Meinke, Stefan
Goldammer, Gesine
Strauch, Miriam
Schubert, Daniel
Timmermann, Bernd
Heyd, Florian
Preußner, Marco
- DOI
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10.15252/embr.202051369
- URN
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urn:nbn:de:101:1-2022070408083254364741
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:28 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Neumann, Alexander
- Meinke, Stefan
- Goldammer, Gesine
- Strauch, Miriam
- Schubert, Daniel
- Timmermann, Bernd
- Heyd, Florian
- Preußner, Marco
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Noisy splicing, more than expression regulation, explains why some exons are subject to nonsense-mediated mRNA decay
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Post-transcriptional regulation of 5-lipoxygenase mRNA expression via alternative splicing and nonsense-mediated mRNA decay
Mammalian tissues defective in nonsense-mediated mRNA decay display highly aberrant splicing patterns
Intergenic splicing-stimulated transcriptional readthrough is suppressed by nonsense-mediated mRNA decay in Arabidopsis
The splicing factor SRSF3 is functionally connected to the nuclear RNA exosome for intronless mRNA decay
RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway
Noisy splicing, more than expression regulation, explains why some exons are subject to nonsense-mediated mRNA decay
Regulatory mechanisms of incomplete huntingtin mRNA splicing
GIGYF recruits mRNA decay factors to repress target mRNA expression
SRSF2 is required for mRNA splicing during spermatogenesis
Nonsense-mediated mRNA decay in collagen X
YBX1 mediates alternative splicing and maternal mRNA decay during pre-implantation development
Post-transcriptional regulation of 5-lipoxygenase mRNA expression via alternative splicing and nonsense-mediated mRNA decay
Post-transcriptional regulation of 5-lipoxygenase mRNA expression via alternative splicing and nonsense-mediated mRNA decay
Mammalian tissues defective in nonsense-mediated mRNA decay display highly aberrant splicing patterns
Intergenic splicing-stimulated transcriptional readthrough is suppressed by nonsense-mediated mRNA decay in Arabidopsis
The splicing factor SRSF3 is functionally connected to the nuclear RNA exosome for intronless mRNA decay
RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway
Noisy splicing, more than expression regulation, explains why some exons are subject to nonsense-mediated mRNA decay
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GIGYF recruits mRNA decay factors to repress target mRNA expression
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