Funktionen des Transkriptionsfaktors c-Jun in der ER-Stressantwort der Leber

Hochschulschrift

Zusammenfassung: Introduction: The accumulation of proteins in the endoplasmic reticulum (ER) and the triggered cellular ER-stress response are associated with cell damage in a wide range of liver diseases. However, the involved signaling pathways have not yet been adequately characterized. Preliminary work has shown that the transcription factor c-Jun/AP-1 posseses important functions in the regulation of survival and proliferation of hepatocytes in acute hepatitis and liver cancer. Objectives: Therefore, here we investigated the functions of c-Jun in the ER-stress response of the liver. Methods: ER-stress was induced in human hepatoma cells either through the chemical ER-stressors Thapsigargin and Tunicamycin or by the expression of hepatoviral proteins. The functions of c-Jun were then determined through RNAi. In addition ER-stress was chemically induced in liver specific conditional c-Jun knockout-mice. Results: In hepatoma cells the induction of ER stress resulted in a rapid and significant expression of c-Jun. Surprisingly, the inhibition of c-Jun expression by RNAi had no effect on cell survival. Primary mouse hepatocytes, however, showed an exacerbation of ER-stress, a vacuolisation of the cytoplasm, a decrease in cell survival and an increased expression of proinflammatory cytokines in the absence of c-Jun. Conclusion: In summary this data shows a protective effect of c-Jun in the context of the ER-stress response and a reduction in the inflammatory response in liver cells under ER-stress conditions by c-Jun

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Deutsch
• Anmerkungen: Albert-Ludwigs-Universität Freiburg, Dissertation, 2011

Klassifikation und Themen

• Schlagwort: Leberstoffwechsel ; Leberkrankheit ; Endoplasmatisches Retikulum ; Oxidativer Stress ; Online-Ressource

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2011
• Urheber: Fuest, Matthias
• Beteiligte Personen und Organisationen: Blum, Hubert E.

Weitere Informationen

• URN: urn:nbn:de:bsz:25-opus-80264
• Rechteinformation: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 14.08.2025, 10:55 MESZ