Topical α-Gal Nanoparticles Enhance Wound Healing in Radiated Skin

Purpose: Surgery within radiated tissue is associated with increased complication rates. It is hypothesized that impaired wound healing may result from aberrant inflammatory responses that occur in previously radiated tissues. Previous work has demonstrated that the topical application of naturally occurring antigen α-gal (Galα1-3Galβ1-(3) 4GlcNAc-R) nanoparticles (AGNs) within wounds accelerates macrophage recruitment and subsequent healing in both normal and diabetic wounds. Herein, we hypothesize that application of this antigen would similarly enhance wound healing in irradiated tissues. Methods: To simulate human physiology, α-1,3-galactosyltransferase knockout (KO) mice were exposed to the antigen to produce anti-α-gal antibodies (anti-Gal). Ten days prior to wounding, the dorsal skin was irradiated with 1 session of 40 Gy. Bilateral dorsal 6-mm splinted full-thickness wounds were created within the radiated skin and treated with 50 µL of AGNs (50 mg/mL) immediately after wounding and again on postoperative day 1. A control KO group underwent similar irradiation and wounding protocols but was treated with phosphate-buffered saline (PBS) vehicle. Wild-type (WT) mice, which do not produce anti-Gal, went through the same irradiation and wounding. Results: Histologic analysis demonstrated enhanced epithelial migration in the radiated/AGN-treated KO wounds, which was significantly elevated in comparison to radiated/PBS-treated KO wounds beginning by day 15 and continuing until the end of the study (p < 0.01). In WT mice, treatment with AGNs showed no effect on epithelial migration. Conclusions: Topical application of AGNs onto irradiated wounds significantly ameliorates the delayed wound healing classically seen in radiated skin and results in faster wound closure with only transient application.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
Topical α-Gal Nanoparticles Enhance Wound Healing in Radiated Skin ; volume:35 ; number:1 ; year:2022 ; pages:31-40 ; extent:10
Skin pharmacology and physiology ; 35, Heft 1 (2022), 31-40 (gesamt 10)

Urheber
Samadi, Arash
Buro, Justin
Dong, Xue
Weinstein, Andrew
Lara, Daniel O.
Celie, Karel-Bart
Wright, Matthew A.
Gadijko, Mariam A.
Galili, Uri
Spector, Jason A.

DOI
10.1159/000518015
URN
urn:nbn:de:101:1-2022011223145828034162
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:37 MESZ

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Beteiligte

  • Samadi, Arash
  • Buro, Justin
  • Dong, Xue
  • Weinstein, Andrew
  • Lara, Daniel O.
  • Celie, Karel-Bart
  • Wright, Matthew A.
  • Gadijko, Mariam A.
  • Galili, Uri
  • Spector, Jason A.

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