Gold Nanoparticles Decorated with Sialic Acid Terminated Bi‐antennary N‐Glycans for the Detection of Influenza Virus at Nanomolar Concentrations †

to related objects

Abstract: Gold nanoparticles decorated with full‐length sialic acid terminated complex bi‐antennary N‐glycans, synthesized with glycans isolated from egg yolk, were used as a sensor for the detection of both recombinant hemagglutinin (HA) and whole influenza A virus particles of the H1N1 subtype. Nanoparticle aggregation was induced by interaction between the sialic acid termini of the glycans attached to gold and the multivalent sialic acid binding sites of HA. Both dynamic light scattering (DLS) and UV/Vis spectroscopy demonstrated the efficiency of the sensor, which could detect viral HA at nanomolar concentrations and revealed a linear relationship between the extent of nanoparticle aggregation and the concentration of HA. UV/Vis studies also showed that these nanoparticles can selectively detect an influenza A virus strain that preferentially binds sialic acid terminated glycans with α(2→6) linkages over a strain that prefers glycans with terminal α(2→3)‐linked sialic acids.

Location
Deutsche Nationalbibliothek Frankfurt am Main
Extent
Online-Ressource
Language
Englisch

Bibliographic citation
Gold Nanoparticles Decorated with Sialic Acid Terminated Bi‐antennary N‐Glycans for the Detection of Influenza Virus at Nanomolar Concentrations † ; volume:4 ; number:6 ; year:2015 ; pages:708-716 ; extent:9
ChemistryOpen ; 4, Heft 6 (2015), 708-716 (gesamt 9)

Creator
Poonthiyil, Vivek
Nagesh, Prashanth T.
Husain, Matloob
Golovko, Vladimir B.
Fairbanks, Antony J.

DOI
10.1002/open.201500109
URN
urn:nbn:de:101:1-2022112306384182201688
Rights
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Last update
15.08.2025, 7:32 AM CEST

Data provider

This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Show original at data provider

Associated

  • Poonthiyil, Vivek
  • Nagesh, Prashanth T.
  • Husain, Matloob
  • Golovko, Vladimir B.
  • Fairbanks, Antony J.

Other Objects (12)

Synthetic Sialosides Terminated with 8‐ N ‐Substituted Sialic Acid as Selective Substrates for Sialidases from Bacteria and Influenza Viruses

Synthetic Sialosides Terminated with 8‐ N ‐Substituted Sialic Acid as Selective Substrates for Sialidases from Bacteria and Influenza Viruses

Synthetic Sialosides Terminated with 8‐ N ‐Substituted Sialic Acid as Selective Substrates for Sialidases from Bacteria and Influenza Viruses

Synthetic Sialosides Terminated with 8‐ N ‐Substituted Sialic Acid as Selective Substrates for Sialidases from Bacteria and Influenza Viruses

Terminated Local Employees

Archivale

Terminated Local Employees

Terminated Personnel, Prien

Archivale

Terminated Personnel, Prien

Terminated Personnel, Prien

Archivale

Terminated Personnel, Prien

Terminated Local Employees

Archivale

Terminated Local Employees

Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

IUPAC HELM Glycans Extension

IUPAC HELM Glycans Extension

Ce=O Terminated CeO 2

Ce=O Terminated CeO 2

Ce=O Terminated CeO 2

Ce=O Terminated CeO 2

Sialic acids : chemistry, metabolism and function

Sialic acids : chemistry, metabolism and function

Sialic acid utilization by Cronobacter sakazakii

Sialic acid utilization by Cronobacter sakazakii

Related objects