Proteomic and Metabolomic Characterization of Human Neurovascular Unit Cells in Response to Methamphetamine

Abstract: The functional state of the neurovascular unit (NVU), composed of the blood–brain barrier and the perivasculature that forms a dynamic interface between the blood and the central nervous system (CNS), plays a central role in the control of brain homeostasis and is strongly affected by CNS drugs. Human primary brain microvascular endothelium, astrocyte, pericyte, and neural cell cultures are often used to study NVU barrier functions as well as drug transport and efficacy; however, the proteomic and metabolomic responses of these different cell types are not well characterized. Culturing each cell type separately, using deep coverage proteomic analysis and characterization of the secreted metabolome, as well as measurements of mitochondrial activity, the responses of these cells under baseline conditions and when exposed to the NVU‐impairing stimulant methamphetamine (Meth) are analyzed. These studies define the previously unknown metabolic and proteomic profiles of human brain pericytes and lead to improved characterization of the phenotype of each of the NVU cell types as well as cell‐specific metabolic and proteomic responses to Meth.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Proteomic and Metabolomic Characterization of Human Neurovascular Unit Cells in Response to Methamphetamine ; volume:4 ; number:9 ; year:2020 ; extent:13 ; Advanced biosystems ; 4, Heft 9 (2020) (gesamt 13)

Beteiligte, Orts- und Zeitangaben

• Urheber: Herland, Anna ; Maoz, Ben M. ; FitzGerald, Edward A. ; Grevesse, Thomas ; Vidoudez, Charles ; Sheehy, Sean P. ; Budnik, Nikita ; Dauth, Stephanie ; Mannix, Robert ; Budnik, Bogdan ; Parker, Kevin Kit ; Ingber, Donald E.

Weitere Informationen

• DOI: 10.1002/adbi.201900230
• URN: urn:nbn:de:101:1-2022061807362033356014
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:20 MESZ