SEC31a‐ATG9a Interaction Mediates the Recruitment of COPII Vesicles for Autophagosome Formation
Abstract: Autophagy plays an important role in determining stem‐cell differentiation. During the osteogenic differentiation of mesenchymal stem cells (MSCs), autophagosome formation is upregulated but the reason is unknown. A long‐standing quest in the autophagy field is to find the membrane origin of autophagosomes. In this study, cytoplasmic coat protein complex II (COPII) vesicles, endoplasmic reticulum‐derived vesicles responsible for the transport of storage proteins to the Golgi, are demonstrated to be a critical source of osteoblastic autophagosomal membrane. A significant correlation between the number of COPII vesicle and the autophagy level is identified in the rat bone tissues. Disruption of COPII vesicles restrained osteogenesis and decreased the number and size of autophagosomes. SEC31a (an outer coat protein of COPII vesicle) is found to be vital to regulate COPII vesicle‐dependent autophagosome formation via interacting with ATG9a of autophagosomal seed vesicles. The interference of Sec31a inhibited autophagosome formation and osteogenesis in vitro and in vivo. These results identified a novel mechanism of autophagosome formation in osteogenic differentiation of stem cells and identified SEC31a as a critical protein that mediates the interplay between COPII and ATG9a vesicles. These findings broaden the understanding of the regulatory mechanism in the osteogenic differentiation of MSCs.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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SEC31a‐ATG9a Interaction Mediates the Recruitment of COPII Vesicles for Autophagosome Formation ; day:03 ; month:10 ; year:2024 ; extent:15
Advanced science ; (03.10.2024) (gesamt 15)
- Creator
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Nie, Jiaming
Ma, Shaoyang
Wu, Linyue
Li, Ye
Cao, Jiao
Li, Meng
Mei, Peter
Cooper, Paul R.
Li, Ang
Pei, Dandan
- DOI
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10.1002/advs.202405127
- URN
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urn:nbn:de:101:1-2410041414339.507874744320
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:20 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Nie, Jiaming
- Ma, Shaoyang
- Wu, Linyue
- Li, Ye
- Cao, Jiao
- Li, Meng
- Mei, Peter
- Cooper, Paul R.
- Li, Ang
- Pei, Dandan
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