Nanoneedles Induce Targeted siRNA Silencing of p16 in the Human Corneal Endothelium
Abstract: Nanoneedles can target nucleic acid transfection to primary cells at tissue interfaces with high efficiency and minimal perturbation. The corneal endothelium is an ideal target for nanoneedle‐mediated RNA interference therapy aimed at enhancing its proliferative capacity, necessary for tissue regeneration. This work develops a strategy for siRNA nanoninjection to the human corneal endothelium. Nanoneedles can deliver p16‐targeting siRNA to primary human corneal endothelial cells in vitro without toxicity. The nanoinjection of siRNA induces p16 silencing and increases cell proliferation, as monitored by ki67 expression. Furthermore, siRNA nanoinjection targeting the human corneal endothelium is nontoxic ex vivo, and silences p16 in transfected cells. These data indicate that nanoinjection can support targeted RNA interference therapy for the treatment of endothelial corneal dysfunction.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Nanoneedles Induce Targeted siRNA Silencing of p16 in the Human Corneal Endothelium ; day:17 ; month:10 ; year:2022 ; extent:12
Advanced science ; (17.10.2022) (gesamt 12)
- Creator
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Maurizi, Eleonora
Martella, Davide Alessandro
Schiroli, Davide
Merra, Alessia
Mustfa, Salman Ahmad
Pellegrini, Graziella
Macaluso, Claudio
Chiappini, Ciro
- DOI
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10.1002/advs.202203257
- URN
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urn:nbn:de:101:1-2022101815483257599438
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
- 15.08.2025, 7:27 AM CEST
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Associated
- Maurizi, Eleonora
- Martella, Davide Alessandro
- Schiroli, Davide
- Merra, Alessia
- Mustfa, Salman Ahmad
- Pellegrini, Graziella
- Macaluso, Claudio
- Chiappini, Ciro
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In vivo evaluation of polymeric siRNA nanoformulations for tumoral gene silencing
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