Immobilization of Amano Lipase A onto Stöber silica surface: process characterization and kinetic studies

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Abstract: The immobilization of Amano Lipase A from Aspergillus niger by adsorption onto Stöber silica matrix obtained by sol-gel method was studied. The effectiveness of the enzyme immobilization and thus the usefulness of the method was demonstrated by a number of physicochemical analysis techniques including Fourier Transform Infrared Spectroscopy (FT-IR), elemental analysis (EA), thermogravimetric analysis (TG), porous structure of the support and the products after immobilization from the enzyme solution with various concentration at different times. The analysis of the process’ kinetics allowed the determination of the sorption parameters of the support and optimization of the process. The optimum initial concentration of the enzyme solution was found to be 5 mg mL-1, while the optimum time of the immobilization was 120 minutes. These values of the variable parameters of the process were obtained by as ensuring the immobilization of the largest possible amount of the biocatalyst at

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
Immobilization of Amano Lipase A onto Stöber silica surface: process characterization and kinetic studies ; volume:13 ; number:1 ; year:2014 ; extent:11
Open chemistry ; 13, Heft 1 (2014) (gesamt 11)

Urheber
Zdarta, Jakub
Sałek, Karina
Kołodziejczak-Radzimska, Agnieszka
Siwińska-Stefańska, Katarzyna
Szwarc-Rzepka, Karolina
Norman, Małgorzata
Klapiszewski, Łukasz
Bartczak, Przemysław
Kaczorek, Ewa
Jesionowski, Teofil

DOI
10.1515/chem-2015-0017
URN
urn:nbn:de:101:1-2410151751312.913121984171
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:23 MESZ

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Beteiligte

  • Zdarta, Jakub
  • Sałek, Karina
  • Kołodziejczak-Radzimska, Agnieszka
  • Siwińska-Stefańska, Katarzyna
  • Szwarc-Rzepka, Karolina
  • Norman, Małgorzata
  • Klapiszewski, Łukasz
  • Bartczak, Przemysław
  • Kaczorek, Ewa
  • Jesionowski, Teofil

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