Electron cryo-microscopy reveals the structure of the archaeal thread filament
Abstract: Pili are filamentous surface extensions that play roles in bacterial and archaeal cellular processes such as adhesion, biofilm formation, motility, cell-cell communication, DNA uptake and horizontal gene transfer. The model archaeaon Sulfolobus acidocaldarius assembles three filaments of the type-IV pilus superfamily (archaella, archaeal adhesion pili and UV-inducible pili), as well as a so-far uncharacterised fourth filament, named “thread”. Here, we report on the cryo-EM structure of the archaeal thread. The filament is highly glycosylated and consists of subunits of the protein Saci_0406, arranged in a head-to-tail manner. Saci_0406 displays structural similarity, but low sequence homology, to bacterial type-I pilins. Thread subunits are interconnected via donor strand complementation, a feature reminiscent of bacterial chaperone-usher pili. However, despite these similarities in overall architecture, archaeal threads appear to have evolved independently and are likely assembled by a distinct mechanism
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
Nature communications. - 13, 1 (2022) , 7411, ISSN: 2041-1723
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2023
- Urheber
-
Gaines, Matthew C.
Isupov, Michail N.
Sivabalasarma, Shamphavi
Haque, Risat Ul
McLaren, Mathew
Mollat, Clara L.
Tripp, Patrick
Neuhaus, Alexander
Gold, Vicki A. M.
Albers, Sonja Verena
Daum, Bertram
- DOI
-
10.1038/s41467-022-34652-4
- URN
-
urn:nbn:de:bsz:25-freidok-2326200
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:25 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Gaines, Matthew C.
- Isupov, Michail N.
- Sivabalasarma, Shamphavi
- Haque, Risat Ul
- McLaren, Mathew
- Mollat, Clara L.
- Tripp, Patrick
- Neuhaus, Alexander
- Gold, Vicki A. M.
- Albers, Sonja Verena
- Daum, Bertram
- Universität
Entstanden
- 2023
Ähnliche Objekte (12)
Electron cryo-microscopy reveals the structure of the archaeal thread filament
Editorial: Archaeal cell envelope and surface structures
CryoEM reveals the structure of an archaeal pilus involved in twitching motility
Wie ein rotierender Typ-IV-Pilus Archaeen das Schwimmen beibrachte
The archaeal protein SepF is essential for cell division in Haloferax volcanii
Putative nucleotide-based second messengers in the archaeal model organisms Haloferax volcanii and Sulfolobus acidocaldarius
Enzymatic asymmetric reduction of unfunctionalized C=C Bonds with archaeal geranylgeranyl reductases
Protein phosphorylation and its role in archaeal signal transduction
The archaeal triphosphate tunnel metalloenzyme SaTTM defines structural determinants for the diverse activities in the CYTH protein family
Diversity and evolution of type IV pili systems in archaea
A comprehensive history of motility and Archaellation in Archaea
Two membrane-bound transcription factors regulate expression of various type-IV-pili surface structures in Sulfolobus acidocaldarius
Electron cryo-microscopy reveals the structure of the archaeal thread filament
Editorial: Archaeal cell envelope and surface structures
CryoEM reveals the structure of an archaeal pilus involved in twitching motility
Wie ein rotierender Typ-IV-Pilus Archaeen das Schwimmen beibrachte
The archaeal protein SepF is essential for cell division in Haloferax volcanii
Putative nucleotide-based second messengers in the archaeal model organisms Haloferax volcanii and Sulfolobus acidocaldarius
Enzymatic asymmetric reduction of unfunctionalized C=C Bonds with archaeal geranylgeranyl reductases
Protein phosphorylation and its role in archaeal signal transduction
The archaeal triphosphate tunnel metalloenzyme SaTTM defines structural determinants for the diverse activities in the CYTH protein family
Diversity and evolution of type IV pili systems in archaea
A comprehensive history of motility and Archaellation in Archaea
Two membrane-bound transcription factors regulate expression of various type-IV-pili surface structures in Sulfolobus acidocaldarius
Electron cryo-microscopy reveals the structure of the archaeal thread filament
Editorial: Archaeal cell envelope and surface structures
CryoEM reveals the structure of an archaeal pilus involved in twitching motility
Wie ein rotierender Typ-IV-Pilus Archaeen das Schwimmen beibrachte
The archaeal protein SepF is essential for cell division in Haloferax volcanii
Putative nucleotide-based second messengers in the archaeal model organisms Haloferax volcanii and Sulfolobus acidocaldarius
Enzymatic asymmetric reduction of unfunctionalized C=C Bonds with archaeal geranylgeranyl reductases
Protein phosphorylation and its role in archaeal signal transduction
The archaeal triphosphate tunnel metalloenzyme SaTTM defines structural determinants for the diverse activities in the CYTH protein family
Diversity and evolution of type IV pili systems in archaea
A comprehensive history of motility and Archaellation in Archaea