Specific Spatial Localization of Actin and DNA in a Water/Water Microdroplet: Self‐Emergence of a Cell‐Like Structure
Abstract: The effect of binary hydrophilic polymers on a pair of representative bio‐macromolecules in a living cell has been examined. The results showed that these bio‐macromolecules exhibited specific localization in cell‐sized droplets that were spontaneously formed through water/water microphase segregation under crowding conditions with coexisting polymers. In these experiments, a simple binary polymer system with poly (ethylene glycol) (PEG) and dextran (DEX) was used. Under the conditions of microphase segregation, DNA was entrapped within cell‐sized droplets rich in DEX. Similarly, F‐actin, linearly polymerized actin, was entrapped specifically within microdroplets rich in DEX, whereas G‐actin, a monomeric actin, was distributed evenly inside and outside these droplets. This study has been extended to a system with both F‐actin and DNA, and it was found that DNA molecules were localized separately from aligned F‐actin proteins to create microdomains inside microdroplets, reflecting the self‐emergence of a cellular morphology similar to a stage of cell division.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Specific Spatial Localization of Actin and DNA in a Water/Water Microdroplet: Self‐Emergence of a Cell‐Like Structure ; volume:19 ; number:13 ; year:2018 ; pages:1370-1374 ; extent:5
ChemBioChem ; 19, Heft 13 (2018), 1370-1374 (gesamt 5)
- Creator
-
Nakatani, Naoki
Sakuta, Hiroki
Hayashi, Masahito
Tanaka, Shunsuke
Takiguchi, Kingo
Tsumoto, Kanta
Yoshikawa, Kenichi
- DOI
-
10.1002/cbic.201800066
- URN
-
urn:nbn:de:101:1-2022090306405426903640
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:30 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Nakatani, Naoki
- Sakuta, Hiroki
- Hayashi, Masahito
- Tanaka, Shunsuke
- Takiguchi, Kingo
- Tsumoto, Kanta
- Yoshikawa, Kenichi
Other Objects (12)
Biased localization of actin binding proteins by actin filament conformation
A distinct metabolic state arises during the emergence of 2‐cell‐like cells
Emergence of periodic circumferential actin cables from the anisotropic fusion of actin nanoclusters during tubulogenesis
Timing the first emergence and disappearance of global water scarcity
T cell migration requires ion and water influx to regulate actin polymerization
Water, frisch Water
"Water! Water! Everywhere!"
"Water, water, everywhere!"
Simulation of the emergence of cell-like morphologies with evolutionary potential based on virtual molecular interactions
Validity of matched-field source localization in under-ice shallow water
Turnover and localization of the actin-binding protein Drebrin in neurons
Revealing water’s secrets: deuterium depleted water
Biased localization of actin binding proteins by actin filament conformation
A distinct metabolic state arises during the emergence of 2‐cell‐like cells
Emergence of periodic circumferential actin cables from the anisotropic fusion of actin nanoclusters during tubulogenesis
Timing the first emergence and disappearance of global water scarcity
T cell migration requires ion and water influx to regulate actin polymerization
Water, frisch Water
"Water! Water! Everywhere!"
"Water, water, everywhere!"
Simulation of the emergence of cell-like morphologies with evolutionary potential based on virtual molecular interactions
Validity of matched-field source localization in under-ice shallow water
Turnover and localization of the actin-binding protein Drebrin in neurons
Revealing water’s secrets: deuterium depleted water
Biased localization of actin binding proteins by actin filament conformation
A distinct metabolic state arises during the emergence of 2‐cell‐like cells
Emergence of periodic circumferential actin cables from the anisotropic fusion of actin nanoclusters during tubulogenesis
Timing the first emergence and disappearance of global water scarcity
T cell migration requires ion and water influx to regulate actin polymerization
Water, frisch Water
"Water! Water! Everywhere!"
"Water, water, everywhere!"
Simulation of the emergence of cell-like morphologies with evolutionary potential based on virtual molecular interactions
Validity of matched-field source localization in under-ice shallow water
Turnover and localization of the actin-binding protein Drebrin in neurons