The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy
Abstract: Macroautophagy/autophagy is a conserved process in eukaryotic cells that mediates the degradation and recycling of intracellular substrates. Proteins encoded by autophagy‐related (ATG) genes are essentially involved in the autophagy process and must be tightly regulated in response to various circumstances, such as nutrient‐rich and starvation conditions. However, crucial transcriptional activators of ATG genes have remained obscure. Here, we identify the RNA polymerase II subunit Rpb9 as an essential regulator of autophagy by a high‐throughput screen of a Saccharomyces cerevisiae gene knockout library. Rpb9 plays a crucial and specific role in upregulating ATG1 transcription, and its deficiency decreases autophagic activities. Rpb9 promotes ATG1 transcription by binding to its promoter region, which is mediated by Gcn4. Furthermore, the function of Rpb9 in autophagy and its regulation of ATG1/ULK1 transcription are conserved in mammalian cells. Together, our results indicate that Rpb9 specifically activates ATG1 transcription and thus positively regulates the autophagy process.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy ; day:14 ; month:09 ; year:2022 ; extent:15
EMBO reports / European Molecular Biology Organization ; (14.09.2022) (gesamt 15)
- Creator
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Huang, Ting
Jiang, Gaoyue
Zhang, Yabin
Lei, Yuqing
Liu, Shiyan
Li, Huihui
Lu, Kefeng
- DOI
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10.15252/embr.202254993
- URN
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urn:nbn:de:101:1-2022091415501922810927
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:34 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Huang, Ting
- Jiang, Gaoyue
- Zhang, Yabin
- Lei, Yuqing
- Liu, Shiyan
- Li, Huihui
- Lu, Kefeng
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Structure of the multi-subunit chloroplast RNA polymerase
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The RNA polymerase II subunit RPB‐9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription
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Pomegranate activates TFEB to promote autophagy-lysosomal fitness and mitophagy
TMEM9 activates Rab9-dependent alternative autophagy through interaction with Beclin1
Lifespan-increasing drug nordihydroguaiaretic acid inhibits p300 and activates autophagy
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Structural studies of RNA polymerase I transcription
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Author Correction: Tomato 26S Proteasome subunit RPT4a regulates ToLCNDV transcription and activates hypersensitive response in tomato
Tumor suppressor Par-4 activates autophagy-dependent ferroptosis
Structure of the multi-subunit chloroplast RNA polymerase
Modeling RNA polymerase competition: the effect of σ-subunit knockout and heat shock on gene transcription level
The RNA polymerase II subunit RPB‐9 recruits the integrator complex to terminate Caenorhabditis elegans piRNA transcription
Transducin activates cGMP phosphodiesterase by trapping inhibitory γ subunit freed reversibly from the catalytic subunit in solution
Pomegranate activates TFEB to promote autophagy-lysosomal fitness and mitophagy
TMEM9 activates Rab9-dependent alternative autophagy through interaction with Beclin1
Lifespan-increasing drug nordihydroguaiaretic acid inhibits p300 and activates autophagy
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