Comprehensive Modular Synthesis of Ganglioside Glycans and Evaluation of their Binding Affinities to Siglec‐7 and Siglec‐9
Abstract: In the present work, bacterial glycosyltransferases are utilized to construct ganglioside glycans in a convergent approach via a sugar‒nucleotide regeneration system and one‐pot multienzyme reactions. Starting from β‐lactoside enables the diversification of both the glycan moieties and the linkages in the lower α‐arm and upper β‐arm. Overall, a comprehensive panel of 24 natural a‐series (GM3, GM2, GM1a, GD1a, GT1a, and fucosyl‐GM1), b‐series (GD3, GD2, GD1b, GT1b, and GQ1b), c‐series (GT3, GT2, GT1c, GQ1c, and GP1c), α‐series (GM1α, GD1aα, and GT1aα), and o‐series (GA2, GA1, GM1b, GalNAc‐GM1b, and GD1c) ganglioside glycans are prepared, which are suitable for biological studies and further applications. Moreover, a microarray is constructed with these synthesized ganglioside glycans to investigate their binding specificity with recombinant Fc‐fused Siglec‐7 and Siglec‐9, which are immune checkpoint‐like glycan recognition proteins on natural killer cells. The microarray binding results reveal that GD3 and GT1aα are specific ligands for Siglec‐7 and Siglec‐9, respectively, and this discovery can lead to the identification of appropriate ligands for investigating the roles of these Siglecs in immunomodulation.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Comprehensive Modular Synthesis of Ganglioside Glycans and Evaluation of their Binding Affinities to Siglec‐7 and Siglec‐9 ; day:18 ; month:11 ; year:2024 ; extent:10
Advanced science ; (18.11.2024) (gesamt 10)
- Urheber
-
Adak, Avijit K.
Tseng, Hsin‐Kai
Chang, Shu‐Yen
Chiang, Yu‐Ching
Lyu, Ke‐Hong
Lee, Yun‐Sheng
Lu, Wen
Kuo, Wen‐Hua
Angata, Takashi
Lin, Chun‐Cheng
- DOI
-
10.1002/advs.202412815
- URN
-
urn:nbn:de:101:1-2411191311534.146674648068
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:38 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Adak, Avijit K.
- Tseng, Hsin‐Kai
- Chang, Shu‐Yen
- Chiang, Yu‐Ching
- Lyu, Ke‐Hong
- Lee, Yun‐Sheng
- Lu, Wen
- Kuo, Wen‐Hua
- Angata, Takashi
- Lin, Chun‐Cheng
Ähnliche Objekte (12)
Die Rolle von Siglec-H und Siglec-15 in Infektionen und Autoimmunität
Coevolution of Siglec-11 and Siglec-16 via gene conversion in primates
Contribution of SIGLEC-11 and SIGLEC-16 receptors to neuro-inflammation and neurodegeneration
Microglial sialic-acid-binding immunoglobulin-like lectin-H (Siglec-H) and Siglec-11 in neuroinflammation
Normalization cancer immunotherapy: blocking Siglec-15!
Engagement of sialylated glycans with Siglec receptors on suppressive myeloid cells inhibits anticancer immunity via CCL2
Study of human specific microglial receptor siglec-11 and generation of transgenic mice expressing human siglec 11
Ly6D+ Siglec-H+ precursor cells contribute to conventional dendritic cells via a Siglec-H+ Zbtb46+ Ly6D+ intermediary stage
Der Zusammenhang der Expressionsstärke von Siglec-7 und Siglec-9 mit der Krankheitsaktivität in Patienten mit Rheumatoider Arthritis
Sialic Acids on Tumor Cells Modulate IgA Therapy by Neutrophils via Inhibitory Receptors Siglec-7 and Siglec-9
Siglec-14 Enhances NLRP3-Inflammasome Activation in Macrophages
Rolle von Galectinen und Siglec-8 beim Mammakarzinom
Die Rolle von Siglec-H und Siglec-15 in Infektionen und Autoimmunität
Coevolution of Siglec-11 and Siglec-16 via gene conversion in primates
Contribution of SIGLEC-11 and SIGLEC-16 receptors to neuro-inflammation and neurodegeneration
Microglial sialic-acid-binding immunoglobulin-like lectin-H (Siglec-H) and Siglec-11 in neuroinflammation
Normalization cancer immunotherapy: blocking Siglec-15!
Engagement of sialylated glycans with Siglec receptors on suppressive myeloid cells inhibits anticancer immunity via CCL2
Study of human specific microglial receptor siglec-11 and generation of transgenic mice expressing human siglec 11
Ly6D+ Siglec-H+ precursor cells contribute to conventional dendritic cells via a Siglec-H+ Zbtb46+ Ly6D+ intermediary stage
Der Zusammenhang der Expressionsstärke von Siglec-7 und Siglec-9 mit der Krankheitsaktivität in Patienten mit Rheumatoider Arthritis
Sialic Acids on Tumor Cells Modulate IgA Therapy by Neutrophils via Inhibitory Receptors Siglec-7 and Siglec-9
Siglec-14 Enhances NLRP3-Inflammasome Activation in Macrophages
Rolle von Galectinen und Siglec-8 beim Mammakarzinom
Die Rolle von Siglec-H und Siglec-15 in Infektionen und Autoimmunität
Coevolution of Siglec-11 and Siglec-16 via gene conversion in primates
Contribution of SIGLEC-11 and SIGLEC-16 receptors to neuro-inflammation and neurodegeneration
Microglial sialic-acid-binding immunoglobulin-like lectin-H (Siglec-H) and Siglec-11 in neuroinflammation
Normalization cancer immunotherapy: blocking Siglec-15!
Engagement of sialylated glycans with Siglec receptors on suppressive myeloid cells inhibits anticancer immunity via CCL2
Study of human specific microglial receptor siglec-11 and generation of transgenic mice expressing human siglec 11
Ly6D+ Siglec-H+ precursor cells contribute to conventional dendritic cells via a Siglec-H+ Zbtb46+ Ly6D+ intermediary stage
Der Zusammenhang der Expressionsstärke von Siglec-7 und Siglec-9 mit der Krankheitsaktivität in Patienten mit Rheumatoider Arthritis
Sialic Acids on Tumor Cells Modulate IgA Therapy by Neutrophils via Inhibitory Receptors Siglec-7 and Siglec-9
Siglec-14 Enhances NLRP3-Inflammasome Activation in Macrophages